Featured Products

  • EASY Isotyping and IgG Quantification ELISAs

    New! EZ ELISA for Isotyping  

    Mouse Immunoglobulin EASY Isotyping Kit

    The Mouse IgG EASY Isotyping Kit provides a simple, rapid method (2-step ELISA) to characterize mouse monoclonal antibody isotypes in cell culture supernatants or purified antibody preparations.
    Specificity: The method enables the detection of Light chains (Kappa and Lambda), and Heavy chains (Gamma 1, Gamma 2a, Gamma 2b, Gamma 3, and Mu) of mouse immunoglobulin.

    The kit includes ready-to-use reagents necessary to analyze 12 samples in less than 30 minutes. Buffer solutions are color coded in order to simplify pipetting steps. The method employs the quantitative sandwich enzyme immunoassay technique. Anti-mouse antibodies specific to each of the common light and heavy chains are pre-coated in the wells. Samples are pipetted into microwells and Ig present in the sample are bound by the capture antibody. Then, a HRP (horseradish peroxidase) conjugated anti-mouse IgG (H+L) antibody is pipetted and incubated simultaneously with samples. After washing microwells in order to remove any non specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops if the specific immunoglobulin is present in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.

    Cat. No. EZ Mouse Isotyping ELISA Quantity  Price
    IS001A Mouse Immunoglobulin EASY Isotyping Test Kit 1 x 12 tests  $    250
    IS001B Mouse Immunoglobulin EASY Isotyping Test Kit 5 x 12 tests  $    575

    Typical Result:

    ISOresult

    Characterization of an IgG1 antibody: yellow color is observed in well A (corresponding to IgG1 Heavy chain), well F (corresponding to Kappa Light chain), and well H (for the positive control H+L). The well H has to be positive in order to validate the method.


     

    New! EZ ELISAs for IgG Quantification

    Features

    - No reagent preparation
    - User-friendly system and easy handling
    - Divisible strips of 8 wells
    - Results in 30 minutes, only one incubation step
    - Color-coded reagents eliminate operator error
    - A wide dynamic range (from 4 to 2000 ng/ml)

    Applications

    - Monoclonal antibodies production monitoring
    - Screening of therapeutic antibodies
    - Selection of high-producing clones
    - Vectors validation for transfection
    - Contaminants detection

    Cat. No. EZ IgG Quantification ELISAs Quantity  Price
    IS002A Human IgG Easy Quantification ELISA Kit 1 x 96 tests  $    495
    IS002B Human IgG Easy Quantification ELISA Kit 10 x 96 tests  $ 2,425
    IS003A Mouse IgG Easy Quantification ELISA Kit 1 x 96 tests  $    495
    IS003B Mouse IgG Easy Quantification ELISA Kit 10 x 96 tests  $ 2,425
    IS004A Rat IgG Easy Quantification ELISA Kit 1 x 96 tests  $    495
    IS004B Rat IgG Easy Quantification ELISA Kit 10 x 96 tests  $ 2,425
    IS005 Bovine IgG Easy Quantification ELISA Kit 1 x 96 tests  $    495

    The simple EZ ELISA procedure is outlined in the steps below.

    Pre-coated plate with the Mouse Anti IgG capture antibody:

    ISO1

    Step 1:  Standards are pipetted into the microwells. The range of 6 standards is applied to the plate wells.

    ISO2

     

    The diluted samples are pipetted into wells.

    ISOSamples

     

    The secondary antibody, HRP-conjugated anti-mouse IgG antibody is immediately pipetted into microwells.

    ISOHRPAB

     

    The plate is incubated for 15 minutes at room temperature, and then washed.

    After washing, substrate solution (TMB) is added to the microwells.

    ISOTMB

     

    After a 10 minute incubation at RT, Stop Solution is added to the wells.

    ISOStop

    The ELISA is completed in only 30 minutes.

     

    Measurement of absorbance and results analysis:

    ISOAnalysis

    Typical Data

    ISOData

    For a limited time receive a free Sport Water Bottle with your first order of EZ ELISA Mouse Isotyping Kits or any of our new Human, Mouse, Rat, or Bovine IgG Quantification Kits. Mention the code EZ ELISA when you place your order.

  • Matched Antibody Pairs for ELISA

    These high quality mouse anti-human monoclonal antibodies are tested for use in ELISA applications.

    Molecule: Catalog No: Description: Size:
    CD25 / IL-2Ra Capture:     CDM394 Mouse Anti-Human CD25 / IL-2R alpha Clone B-G3 mAb 1.0 mg
    Capture:     CDM005 Mouse Anti-Human CD25 / IL-2R alpha Clone B-G3 mAb 200 μg
    Detection: CDM418

     

    Mouse Anti-Human CD25 / IL-2R alpha Clone B-F2 Biotin mAb

     

    100 μg

     

    CD141 / THBD Capture:     CDM393 Mouse Anti-Human CD141/TMBD Clone B-A35 mAb 1.0 mg
    Capture:     CDM212 Mouse Anti-Human CD141/TMBD Clone B-A35 mAb 200 μg
    Detection: CDM417

     

    Mouse Anti-Human CD141/TMBD Clone B-S24 Biotin mAb

     

    100 μg

     

    CXCL10 / IP-10 Capture:     CDM411 Mouse Anti-Human CXCL10/IP-10 Clone B-C50 mAb 1.0 mg
    Capture:     CDM083 Mouse Anti-Human CXCL10/IP-10 Clone B-C50 mAb 500 μg
    Detection: CDM435

     

    Mouse Anti-Human CXCL10/IP-10 Clone B-C55 Biotin mAb

     

    100 μg

     

    SELE / CD62E Capture:     CDM398 Mouse Anti-Human E-Selectin/CD62E Clone B-P7 mAb 1.0 mg
    Capture:     CDM049 Mouse Anti-Human E-Selectin/CD62E Clone B-P7 mAb 200 μg
    Detection: CDM422

     

    Mouse Anti-Human E-Selectin/CD62E Clone B-S3 Biotin mAb

     

    100 μg

     

    ICAM-1 / CD54 Capture:     CDM397 Mouse Anti-Human ICAM-1/CD54 Clone B-H17 mAb 1.0 mg
    Capture:     CDM045 Mouse Anti-Human ICAM-1/CD54 Clone B-H17 mAb 200 μg
    Detection: CDM421

     

    Mouse Anti-Human ICAM-1/CD54 Clone B-H22 Biotin mAb

     

    100 μg

     

    IFN-gamma Capture:     CDM400 Mouse Anti-Human IFN-gamma Clone B-B1 mAb 1.0 mg
    Capture:     CDM256 Mouse Anti-Human IFN-gamma Clone B-B1 mAb 500 μg
    Detection: CDM424

     

    Mouse Anti-Human IFN-gamma Clone B-G1 Biotin mAb

     

    100 μg

     

    IL-4 Capture:     CDM401 Mouse Anti-Human IL-4 Clone B-R14 mAb 1.0 mg
    Capture:     CDM104 Mouse Anti-Human IL-4 Clone B-R14 mAb 500 μg
    Detection: CDM425

     

    Mouse Anti-Human IL-4 Clone B-G28 Biotin mAb

     

    100 μg

     

    IL-6 Capture:     CDM402 Mouse Anti-Human IL-6 Clone B-E8 mAb 1.0 mg
    Capture:     CDM265 Mouse Anti-Human IL-6 Clone B-E8 mAb 500 μg
    Detection: CDM426

     

    Mouse Anti-Human IL-6 Clone B-E4 Biotin mAb

     

    100 μg

     

    IL-6R / CD126 Capture:     CDM395 Mouse Anti-Human IL-6R Clone B-N12 mAb 1.0 mg
    Capture:     CDM010 Mouse Anti-Human IL-6R Clone B-N12 mAb 200 μg
    Detection: CDM419

     

    Mouse Anti-Human IL-6R Clone B-R6 Biotin mAb

     

    100 μg

     

    IL-6ST / CD130 Capture:     CDM396 Mouse Anti-Human IL-6ST Clone B-P4 mAb 1.0 mg
    Capture:     CDM017 Mouse Anti-Human IL-6ST Clone B-P4 mAb 200 μg
    Detection: CDM420

     

    Mouse Anti-Human IL-6ST Clone B-T2 Biotin mAb

     

    100 μg

     

    IL-7 Capture:     CDM403 Mouse Anti-Human IL-7 Clone B-N18 mAb 1.0 mg
    Capture:     CDM097 Mouse Anti-Human IL-7 Clone B-N18 mAb 500 μg
    Detection: CDM427

     

    Mouse Anti-Human IL-7 Clone B-S16 Biotin mAb

     

    100 μg

     

    IL-12 / p40+p70 Capture:     CDM406 Mouse Anti-Human IL-12 p40 + p70 Clone B-P40 mAb 1.0 mg
    Capture:     CDM281 Mouse Anti-Human IL-12 p40 + p70 Clone B-P40 mAb 500 μg
    Detection: CDM430

     

    Mouse Anti-Human IL-12 p40 + p70 Clone B-P24 Biotin mAb

     

    100 μg

     

    IL-12 / p35+p70 Capture:     CDM405 Mouse Anti-Human IL-12 p35 + p70 Clone B-T21 mAb 1.0 mg
    Capture:     CDM271 Mouse Anti-Human IL-12 p35 + p70 Clone B-T21 mAb 500 μg
    Detection: CDM429

     

    Mouse Anti-Human IL12 p70 Clone B-P24 Biotin mAb

     

    100 μg

     

    IL-13 Capture:     CDM407 Mouse Anti-Human IL-13 Clone B-B13 mAb 1.0 mg
    Capture:     CDM275 Mouse Anti-Human IL-13 Clone B-B13 mAb 500 μg
    Detection: CDM431

     

    Mouse Anti-Human IL-13 Clone B-P6 Biotin mAb

     

    100 μg

     

    IL-17A Capture:     CDM408 Mouse Anti-Human IL-17A Clone B-C49 mAb 1.0 mg
    Capture:     CDM120 Mouse Anti-Human IL-17A Clone B-C49 mAb 500 μg
    Detection: CDM432

     

    Mouse Anti-Human IL-17A Clone B-B51 Biotin mAb

     

    100 μg

     

    IL-17F Capture:     CDM416 Mouse Anti-Human IL-17F Clone B-G46 mAb 1.0 mg
    Capture:     CDM123 Mouse Anti-Human IL-17F Clone B-G46 mAb 500 μg
    Detection: CDM450

     

    Mouse Anti-Human IL-17F Clone B-F60 Biotin mAb

     

    100 μg

     

    IL-23A Capture:     CDM409 Mouse Anti-Human IL-23A Clone B-Z23 mAb 1.0 mg
    Capture:     CDM115 Mouse Anti-Human IL-23A Clone B-Z23 mAb 500 μg
    Detection: CDM433

     

    Mouse Anti-Human IL-23A Clone B-F43 Biotin mAb

     

    100 μg

     

    IL-27 Capture:     CDM410 Mouse Anti-Human IL-27 Clone B-G44 mAb 1.0 mg
    Capture:     CDM122 Mouse Anti-Human IL-27 Clone B-G44 mAb 500 μg
    Detection: CDM434

     

    Mouse Anti-Human IL-27 Clone B-G49 Biotin mAb

     

    100 μg

     

    p53 Capture:     CDM413 Mouse Anti-Human p53 Clone B-F34 mAb 1.0 mg
    Capture:     CDM091 Mouse Anti-Human p53 Clone B-F34 mAb 200 μg
    Detection: CDM437

     

    Mouse Anti-Human p53 Clone B-P3 Biotin mAb

     

    100 μg

     

    Perforin Capture:     CDM414 Mouse Anti-Human Perforin Clone B-E48 mAb 1.0 mg
    Capture:     CDM090 Mouse Anti-Human Perforin Clone B-E48 mAb 200 μg
    Detection: CDM438

     

    Mouse Anti-Human Perforin Clone B-D48 Biotin mAb

     

    100 μg

     

    TNF-alpha Capture:     CDM412 Mouse Anti-Human TNF-alpha Clone B-F7 mAb 1.0 mg
    Capture:     CDM287 Mouse Anti-Human TNF-alpha Clone B-F7 mAb 500 μg
    Detection: CDM436

     

    Mouse Anti-Human TNF-alpha Clone B-C7 Biotin mAb

     

    100 μg

     

    TRAIL / CD253 Capture:     CDM415 Mouse Anti-Human TRAIL/APO2L Clone B-S23 mAb 1.0 mg
    Capture:     CDM076 Mouse Anti-Human TRAIL/APO2L Clone B-S23 mAb 200 μg
    Detection: CDM439

     

    Mouse Anti-Human TRAIL/APO2L Clone B-T24 Biotin mAb

     

    100 μg

     

    VCAM-1 / CD106 Capture:     CDM399 Mouse Anti-Human VCAM-1/CD106 Clone B-S6 mAb 1.0 mg
    Capture:     CDM106 Mouse Anti-Human VCAM-1/CD106 Clone B-S6 mAb 200 μg
    Detection: CDM423 Mouse Anti-Human VCAM-1/CD106 Clone B-N8 Biotin mAb 100 μg