Whitepaper: LBP as an Indicator of Disease States

Lipopolysaccharide-Binding Protein (LBP) as an Indicator of Disease States in Multiple Species

Amanda Flies, Ph.D. and Thomas H. Ermak, Ph.D.

LBP-activity

LBP binds multimers of LPS in the blood or other bodily fluids and transfers LPS monomers to CD14 on the surface of an innate immune cell. LPS is detected at the cell’s surface by a protein complex involving CD14, MD2 and TLR4, which signals through its intracellular domain. Recognition of bacterial products such as LPS may activate innate immune cells to produce inflammatory mediators.

Abstract
Lipopolysaccharide binding protein (LBP) is a 58-kD glycoprotein involved in the acute-phase immunologic response to Gram-negative bacterial infections. It binds with high affinity to the lipid A portion of lipopolysaccharide (LPS), which Gram-negative bacteria express on their outer membrane. The presence of LBP in blood and bodily fluids is an indicator of bacterial infection, and as such is a useful marker of a number of disease states in humans, cows, mice and other animals.
As a method of detecting this important acute-phase protein, ELISA (enzyme-linked immunosorbent assay) offers specificity and reliable quantitation of the protein levels in samples such as blood, serum, lavage fluid, or milk. The Cell Sciences® Multispecies LBP ELISA kit maintains specificity for human LBP, while also providing flexibility to assess a broad range of LBP homologs in other species.

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LBP Reagents offered by Cell Sciences

CKH113  Human LBP Multispecies Reactive ELISA Kit

CKM043  Mouse LBP ELISA Kit

CML002  Mouse Anti-Human LBP Clone biG 42 mAb†

CML003  Mouse Anti-Human LBP Clone biG 48 mAb†

CML004  Mouse Anti-Human LBP Clone biG 412 mAb*

CML007  Mouse Anti-Human LBP Clone biG 43 mAb†

CPC402A  Rabbit Anti-Human LBP pAb

CML001  Mouse Anti-Mouse LBP Clone biG 33 mAb*

CML005  Mouse Anti-Mouse LBP Clone biG 35 mAb†

CPC105  Rabbit Anti-Mouse LBP pAb

CRL701  Recombinant Human LBP

CRL700  Recombinant Mouse LBP

* Inhibits binding to CD14        † Does not inhibit binding to CD14

 

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