Bovine IgG Easy Quantification ELISA Kit

Molecule:

IgG

Species:

Bovine

Cat. No.:

IS005 pdf (datasheet)

Quantity/Size:

1 x 96 tests

Price:

$495.00 BUY

Description:

The Bovine IgG Easy Quantification Kit provides a rapid and easy method (one antibody step ELISA) for the quantitative determination of bovine IgG in cell culture supernatant and serum. The kit includes ready-to-use reagents necessary to analyze up to 89 samples in 45 min. Buffer solutions are color coded in order to simplify pipetting steps.

Principle of the Assay:
The method employs the quantitative sandwich enzyme immunoassay technique. A polyclonal antibody specific to bovine IgG is pre-coated onto the microwells. Samples and standards are pipetted into microwells, and bovine IgG present in the sample is bound by the capture antibody. Next, a HRP (horseradish peroxidase) conjugated anti-bovine IgG (H+L) antibody is pipetted and incubated simultaneously with samples. After washing the microwells in order to remove any non-specific binding, the ready to use substrate solution (TMB) is added to microwells. Color develops proportionally to the amount of bovine IgG in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.

Specificity:
The method enables the detection of all bovine IgG. No cross reaction was observed with the following species: Mouse and Human.

Sensitivity:
The detection range is from 10 ng/ml to 2000 ng/ml. For antibodies with a concentration of 1 mg/ml, the kit allows the detection of bovine IgG contamination of about 20 ppm.

Data PDF:

IS005

Storage/Stability:

All kit components are stable for 12 months when stored at 2-8°C. Do not freeze.

Application:

ELISA