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CCL4, Human Chemokine Ligand 4 ELISA Kit
 
Catalog No:   CKH169     (datasheet)
Brand:   Cell Sciences
Quantity:   1 x 96 tests
Price:   $495.00   add to cart

Category/Sub:   Kits / ELISA Kits
Description:   The Cell Sciences Human CCL4 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human MIP1-beta in serum, plasma, cell culture supernatants and urine. This assay employs an antibody specific for human CCL4 coated on a 96-well plate. Standards and samples are pipetted into the wells and CCL4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human CCL4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIP1-beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Alternate Names: MIP1-beta, ACT2, LAG1, MIP1B, SCYA2, SCYA4

Specificity: Human CCL4

Sample Types: Serum, plasma, cell culture supernatants, and urine

Sensitivity: < 2.5 pg/ml

Reproducibility: Intra-Assay: CV < 10%
Inter-Assay: CV < 12%

Storage and Stability: Product can be stored for up to 5 months from date of receipt at 2-4°C. Standard (recombinant protein) should be stored at -20°C or -80°C after reconstitution. Opened Microplate Wells and reagents can be stored for up to one month at 2-4°C.



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