The kit contains six pre-coated 8-well strips for each of the four IgG subclasses.
Test description:
The PeliClass human subclass ELISA kit is a 'sandwich'-type enzyme immunoassay. The kit contains microwell strips coated with highly avid monoclonal antibodies, each specific for one of the human IgG subclasses. Test samples, calibrator- and control sera are incubated in the respective wells. The IgG subclass to be determined will bind to the solid phase and non-bound IgG is removed by washing. Next, peroxidase-conjugated anti-human IgG antiserum is added to each well and non-bound conjugate is removed by washing. After incubation with substrate solution (ABTS) and H2O2, the reaction is stopped with an acid buffer. The green colored reaction product is measured by absorbance and the concentration of IgG subclass in the test sample calculated relative to the values of a reference curve.IgG subclass control serum is assayed to check the validity of the calibration curves and the accuracy of the IgG subclass determinations. This assay is highly sensitive and specific and allows for accurate measurement of very low IgG subclass levels.
Kit contents:
The kit contains six pre-coated 8-well strips for each of the four IgG subclasses and additional reagents.
•two pre-coated microtiter plates with six colour-coded 8-well strips for each IgG subclass
•horseradish peroxidase-conjugated anti-human IgG antibodies
•calibration and control sera
•wash-, dilution-, substrate- and stop buffers
•ABTS and hydrogen peroxidase substrate stock solution
•reference values for human IgG subclass concentrations
