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Anti-human Proteinase 3, PR3 Clone WGM2, Monoclonal Antibody
| Catalog No: |
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HM2171
(datasheet)
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| Brand: |
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Hycult Biotechnology |
| Quantity: |
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100 µg |
| Price: |
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$455.00
add to cart
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| Category/Sub: |
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Monoclonal Antibodies / Antimicrobial Peptides Defensins |
| Notes: |
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The monoclonal antibody WGM2 can be used for flow cytometry, Western blotting and immunohistology on both frozen and paraffin embedded sections. Furthermore the antibody WGM2 can be used in immuno assays, for inhibition of biological PR3 activity and induction of monocytes and neutrophils activation. |
| Isotype: |
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Mouse IgG1 |
| Description: |
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Monoclonal antibody WGM2 reacts with human proteinase 3 (PR3), a 30 kDa protein. PR3 is a major antigen recognized by autoantibodies directed against cytoplasmic proteins of neutrophilic granulocytes and monocytes (so called anti-neutrophil cytoplasmic autoantibodies (ANCA)). ANCA are able to activate primed neutrophils to produce oxygen radicals and release lytic enzymes, including PR3. Proteinase 3 (PR3) was identified as the target antigen of ANCA in Wegener’s granulomatosis (WG). ANCA directed against PR3 (PR3-ANCA) can interfere with the binding of PR3 to its physiological inhibitor alpha1-antitrypsin (alpha1-AT) and with the proteolytic activity of PR3. At the site of inflammation PR3 can cleave the complex between these inhibiting ANCA and PR3 itself, leaving active PR3. Antibodies to PR3 are potent inductors of the 5-lipoxygenase pathway in primed human neutrophils, and extracellular free arachidonic acid (AA), as provided at an inflammatory focus, synergizes with the autoantibodies to evoke full-blown lipid mediator generation, granule secretion and respiratory burst.
Proteinase 3 (PR3) is a neutral serine proteinase, which is localized in the azurophilic granules of neutrophils and in granules of monocytes. PR3 degrades a number of extracellular matrix proteins such as elastin and inactivates human C1 inhibitor. Monoclonal antibody WGM2 blocks the PR3 activity and partially inhibits the binding of human PR3-ANCA to PR3.
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FC WB IH IA |
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