Cynomolgus Monkey IgG ELISA kit

Molecule:

IgG

Species:

Cynomolgus Monkey

Cat. No.:

CKK001B pdf (datasheet)

Quantity/Size:

5 x 96 tests

Price:

$2,450.00 BUY

Description:

Intended Use: This cynomolgus macaque (Macaca fascicularis) monkey Immunoglobulin G (IgG) antigen assay is intended for the quantitative determination of total IgG antigen in cyno monkey serum, plasma, hybridoma cell supernatants, ascites or other biological fluids. The assay does not distinguish IgG subclasses.

Background: IgG is the most abundant immunoglobulin in serum and is predominately involved in the secondary immune response. The IgG subclasses are designated 1, 2, 3 and 4 based on their relative prevalence in human serum.

Assay Principle: Cynomolgus monkey IgG will bind to the monoclonal capture antibody coated on the microtiter plate. After appropriate washing steps, biotin labeled monoclonal anti-Cynomolgus IgG primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with horseradish peroxidase conjugated streptavidin. Following an additional washing step, TMB substrate is used for color development at 450 nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of Cynomolgus monkey IgG. Color development is proportional to the concentration of IgG in the samples.

Data PDF:

CKK001

Storage/Stability:

Store kit components at 4°C. Store unopened plate and any unused microtiter strips in the pouch with desiccant. Reconstituted standards and primary may be stored at -80°C for later use. DO NOT freeze/thaw the standards and primary antibody more than once

Application:

ELISA