Cynomolgus Monkey Plasminogen Total Antigen ELISA Kit

Molecule:

PLG

Species:

Cynomolgus Monkey

Cat. No.:

CKK000A pdf (datasheet)

Quantity/Size:

1 x 96 tests

Price:

$695.00 BUY

Description:

Intended Use: This cynomolgus macaque (Macaca fascicularis) monkey plasminogen total assay is for the quantitative determination of total plasminogen antigen in plasma, serum, or cell culture media. This kit has been formulated for research only.

Background: Plasminogen is a single chain glycoprotein zymogen and is the precursor of the fibrinolytic enzyme plasmin. Plasminogen deficiencies are classified as hypoplasminogenemia (Type I) or dysplasminogenemia (Type 2) and are associated with decreased extracellular fibrin clearance leading to mucous membrane lesions and ligneous conjunctivitis.

Assay Principle: Cynomolgus monkey plasminogen will bind to the affinity purified capture antibody coated on the microtiter plate. Plasminogen, plasmin, and plasmin in complex with antiplasmin will react with the antibody on the plate. After appropriate washing steps, biotin labeled polyclonal anti-cynomolgus plasminogen primary antibody binds to the captured protein. Excess antibody is washed away and bound polyclonal antibody is then reacted with peroxidase conjugated streptavidin. Following an additional washing step, TMB substrate is used for color development at 450 nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of cynomolgus plasminogen. Color development is proportional to the concentration of total plasminogen in the sample.

Data PDF:

CKK000

Storage/Stability:

All kit components must be stored at 4°C. Store unopened plate and any unused microtiter strips in the pouch with desiccant. Reconstituted standards and primary may be stored at -80°C for later use. DO NOT freeze/thaw the standard and primary antibody mo

Application:

ELISA