Human Insulin ELISA Kit

Molecule:

INS

Synonyms:

IDDM , IDDM1 , IDDM2 , ILPR , IRDN , MODY10

Species:

Human

Cat. No.:

CKH345 pdf (datasheet)

Quantity/Size:

1 x 96 tests

Price:

$575.00 BUY

Description:

The Human Insulin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Insulin and Proinsulin in serum, plasma, cell culture supernatants and urine. This assay employs an antibody specific for human Insulin coated on a 96-well plate. Standards and samples are pipetted into the wells and Insulin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Insulin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Insulin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Entrez Symbol:

INS

Data PDF:

CKH345

Kit Plates:

Precoated plates

Storage/Stability:

May be stored for up to 6 months at 2οΨΑ to 8οΨΑC from the date of shipment. Opened Microplate Wells or reagents may be store for up to 1 month at 2οΨΑ to 8οΨΑC. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Reconst