Human LR3 IGF-I Rapid ELISA Kit

Molecule:

IGF1

Species:

Human

Cat. No.:

BEK-2233-1P pdf (datasheet)

Quantity/Size:

1 x 96 tests

Price:

$595.00 BUY

Description:

Human LR³ insulin-like Growth Factor-I (LR³ IGF-I) was developed specifically for supplementation of mammalian cell culture to support the survival and proliferation of cells. It is an 83 amino acid analogue of IGF-I comprising the complete human IGF-I sequence with the substitution of an Arginine for the Glutamine at position 3, plus a 13 amino acid extension peptide at the N-terminus. It is engineered to have a higher biological potency than native IGF-I or IGF-II and has several advantages over recombinant insulin. Supplementation of cell cultures with LR³ IGF-I at a much lower concentration results in equivalent or better productivity than supplementation with standard concentrations of insulin. LR³ IGF-I is better able to stimulate the type I IGF receptor and thus induce a higher level of activation of intracellular signaling molecules, which are responsible for promoting cell survival by inhibition of apoptosis.

The LR³ IGF-I Rapid ELISA kit consists of a complete set of reagents and pre-coated plate to allow immediate assay of LR³ IGF-I in culture media. Included in the kit are mouse monoclonal anti- LR³ IGF-I capture antibody pre-coated onto an ELISA plate, standard LR³ IGF-I protein, a biotinylated anti-LR³ IGF-I detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3’,5,5’-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of LR³ IGF-I present in samples and the supplied protein standard.

The LR³ IGF-I Rapid ELISA kit has been developed, optimized and validated to quantify LR³ IGF-I protein in cell culture medium and is likely to be used during downstream processing of media following a production cycle and is not intended for other use.

Samples: Serum, Plasma, Cell culture supernatants

Reagents Included:

1. 96 well plate coated with LR³ IGF-I Capture Antibody

2. Recombinant LR³ IGF-I Standard

3. Biotinylated LR³ IGF-I Detection Antibody

4. Streptavidin-HRP Concentrate

5. TMB Substrate Reagent

6. Assay Diluent

7. Wash Buffer

8. Stop Solution

9. Plate Sealer

Entrez Symbol:

IGF1

Data PDF:

BEK-2233-1P

Kit Plates:

pre-coated plate

Storage/Stability:

Stable for up to 12 months at 2-8°C

Application:

ELISA