Human Plasmin Antiplasmin Complex Total Antigen ELISA Kit

Species:

Human

Cat. No.:

CKH021B pdf (datasheet)

Quantity/Size:

5 x 96 tests

Price:

$2,765.00 BUY

Description:

Human Plasmin / Antiplasmin (PAP) Complex ELISA assay is intended for the quantitative determination of the covalent complex of plasmin and its inhibitor α2-antiplasmin in human plasma, serum, urine, cell culture supernatants, and tissue extracts. For research use only.

Plasminogen is a single chain glycoprotein zymogen and is the precursor of the fibrinolytic enzyme plasmin. The serine protease plasmin rapidly forms a 1:1 covalent complex with its major circulating inhibitor α2-antiplasmin. Detection of PAP complex in plasma represents activation of the fibrinolytic system either directly or secondarily to coagulation in vivo.

Human PAP complex in samples will bind to the antihuman plasmin capture antibody coated on the microtiter plate. After appropriate washing steps, polyclonal anti-α2-antiplasmin primary antibody binds to PAP complex captured on the plate. Excess antibody is washed away and bound polyclonal antibody is then reacted with horseradish peroxidase conjugated streptavidin. TMB substrate is used for color development at 450 nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of PAP complex. Color development is proportional to the concentration of PAP complex in the samples. Free plasminogen and antiplasmin will not be detected by this assay.

Data PDF:

CKH021

Application:

ELISA