Human Prostate Tissue Lysate

Species:

Human

Cat. No.:

PX468A pdf (datasheet)

Quantity/Size:

100 μg

Price:

$175.00 BUY

Description:

Prostate tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Data PDF:

PX468

Application:

WB

Application notes:

The human prostate tissue lysate is ready to load on SDS-PAGE for Western blotting. It is recommended to load 10 μg to 20 μg per lane for mini gel.

Formulation:

Prostate lysate is supplied in SDS sample buffer containing 5% β-mercaptoethanol.

Storage/Stability:

It is supplied as whole cell lysate, 100 μg per vial at 2.0 mg/ml, in 1 x SDS sample buffer containing 5% β-mercaptoethanol. Storage at -20°C is stable for three months, -70°C for one year.