Mouse Anti-His Tag Clone HIS.H8 mAb

Molecule:

His

Cat. No.:

CSI20563A pdf (datasheet)

Quantity/Size:

50 μg

Price:

$150.00 BUY

Concentration:

1.0 mg/ml

Description:

Anti-His Tag Clone HIS.H8 / EH158 mAb

Data PDF:

CSI20563

Specificity:

Recognizes the epitope of 6x His-tags encoded by many commercially available vectors, regardless of the tag's location in the fusion protein sequences (i.e.: reacts with N-terminal, C-terminal or internal 6x His-tags)

Host Species:

Mouse

Immunogen:

HHHHHH (6x His) synthetic peptide conjugated to KLH

Isotype:

IgG2b

Clone:

HIS.H8 / EH158

Formulation:

Liquid in 10 mM PBS, pH 7.2, + 0.05% NaN3. Precaution: Sodium Azide is a poisonous and hazardous substance which should be handled by trained staff only.

Purification:

Protein A affinity chromatography from mouse ascites fluid

Application notes:

Western blot sensitivity (with ECL): 1-2 ng of His-tagged fusion proteins Wesern Blot (with ECL): working dilution of 1:1,000-3,000 (incubate for one hour at room temperature). Immunostaining: working dilution of 1:500-2,000. The optimal concentration sho

Storage/Stability:

Store at -20οΨΑC for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4οΨΑC for 1-2 weeks.