Mouse Anti-Human CD2 Clone CLB-HIK27 mAb

Molecule:

CD2/LFA-2

Synonyms:

T11; SRBC; LFA-2

Species:

Human

Cat. No.:

M1653 pdf (datasheet)

Quantity/Size:

0.1 ml

Price:

$415.00 BUY

Concentration:

2 mg/ml

Description:

Mouse Anti-Human CD2 Clone CLB-HIK27 monoclonal antibody. This clone has been derived from hybridisation of SP2/0 cells with spleen cells of a (BALB/c x A/J) mouse immunized with cells of a patient with the Sezary Syndrome.

The monoclonal antibody is directed against the CD2 antigen (T11 antigen), which is expressed on human T lymphocytes. The monoclonal antibody reacts with all human peripheral T lymphocytes and 90% of the thymocytes. The monoclonal antibody blocks the rosette formation of human T lymphocytes with sheep erythrocytes.

In general, two signals are required to activate T lymphocytes into proliferation. In vitro, both signals can be given by the proper combination of monoclonal antibodies, in this respect, monoclonal antibodies against CD2, CD3 and CD28 have provided much information on the stimulatory mechanism. It was found that anti-CD2 antibodies are also able to stimulate T cells, although only in the presence of a second signal, which can be given either by a more anti-CD2 antibody directed against other epitopes on the CD2 molecule, and / or e.g. by an anti-CD28 antibody. The binding of anti-CD28 McAbs to T cells was found to enhance stimulation of the cells by anti-CD2 and anti-CD3 mAbs. Therefore, CD28 is regarded as a ‘co-stimulatory’ molecule.

Entrez Symbol:

CD2

Data PDF:

M1653

Specificity:

Human CD2 antigen (T11 antigen), which is expressed on human T lymphocytes

Host Species:

Mouse

Immunogen:

Cells of a patient with the Sezary Syndrome

Isotype:

IgG1

Clone:

CLB-HIK27

Conjugate:

Unconjugated

Formulation:

0.1 ml purified culture supernatant, 0.22 μm filtered, with a concentration of 2 mg/ml in 20 mM TRIS and 150 mM NaCl, pH 8.0.

Application notes:

To induce the proliferation of resting T lymphocytes for further study.

Storage/Stability:

Store at -18 to -32°C. Avoid repeated freeze-thaw cycles.