Mouse Anti-Human Complement C4c Clone 99-72-18 mAb

Molecule:

C4A

Synonyms:

C42, C4A3, C4A4, C4A6, C4AD, C4S, CO4, CPAMD2, RG, C4A

Species:

Human

Cat. No.:

CMH017A pdf (datasheet)

Quantity/Size:

200 µl

Price:

$1,200.00 BUY

Concentration:

1.0 mg/ml

Description:

The mouse monoclonal antibody recognizes human Complement component 4 c (C4c), a component in the complement cascade and a degradation product of C4b which is cleaved by C4b/C3b inactivator to yield C4d and C4c. C4c consists of a gamma-chain (30 kDa), a beta-chain (75 kDa), and two alpha-domains denoted alpha 3 (27 kDa) and alpha 4 (14 kDa). C4c reacts with C1 and C2 to form C3 convertase of the classic activation pathway. The C4 protein derives from the C4A-C4B genes.

ELISA: This antibody may be used as a capture antibody in combination with CMH043 as detection antibody.

Entrez Symbol:

C4A/C4B

Data PDF:

CMH017

Specificity:

Human Complement C4c

Host Species:

Mouse

Immunogen:

Synthetic peptide consisting of the last 15C-terminal residues of the C4c alpha 4-domain (Cys-EELVYELNPLDHRGR).

Isotype:

IgG2BK

Clone:

Clone 99-72-18

Conjugate:

Unconjugated

Formulation:

Liquid in 10 mM phosphate buffer, pH 7.4, + 0.5 M sodium chloride + 15 mM sodium azide. Precaution: Sodium azide is a poisonous and hazardous substance which should be handled by trained staff only.

Purification:

Protein A Chromatography

Application:

ELISA, IF

Application notes:

ELISA: 4 μg/ml as a capture antibody, with biotinylated anti-C4 (CMH043) at 0.4 μg/ml as detection antibody.
IHC: 1:600 for formalin-fixed, paraffin-embedded tissue.
IF: 1:200.