Mouse Anti-Human IL-6 Clone B-E8 Azide Free mAb

Molecule:

IL-6

Synonyms:

CDF; HGF; HSF; BSF2; IL-6; BSF-2; IFNB2; IFN-beta-2

Species:

Human

Cat. No.:

CDM265 pdf (datasheet)

Quantity/Size:

500 μg

Price:

$540.00 BUY

Concentration:

1 mg/ml

Description:

Mouse Anti-Human IL-6 Clone B-E8 Azide Free mAb

This antibody can be used as Capture Antibody in a human IL-6 sandwich immunoassay to detect human IL-6 in combination with biotinylated human IL-6 Detection Antibody (Cat No CDM426). 

Background: Interleukin 6 is a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Alternative splicing results in multiple transcript variants.

Entrez Symbol:

IL6

Uniprot Name:

Interleukin-6

Uniprot ID:

P05231

Data PDF:

CDM265

Specificity:

Human | Recognizes both natural and recombinant human IL-6

Host Species:

Mouse

Immunogen:

Recombinant human IL-6

Isotype:

IgG1

Clone:

B-E8

Conjugate:

Unconjugated

Formulation:

Phosphate-buffered saline. Sterile-filtered through 0.22 μm and treated to remove endotoxins. Carrier and preservative free.

Purification:

Ion exchange chromatography

Application:

ELISA

Application notes:

Blocks IL-6 induced proliferation on B9 cells. Inhibits IL-6 binding to its receptor.
Suitable for intra-cytoplasmic flow cytometry.
Capture Antibody: ELISA: 0.5-2.5 µg/ml, ELISPOT: 5-10 µg/ml

Storage/Stability:

Store at +2-8°C for 12 months. For longer storage, freeze aliquots.