Mouse Anti-Human p53 Clone B-F34 Azide Free Capture mAb

Molecule:

p53/TP53

Synonyms:

P53; BCC7; LFS1; TRP53

Species:

Human

Cat. No.:

CDM413 pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$995.00 BUY

Concentration:

1.0 mg / 1.0 ml

Description:

Mouse Anti-Human p53 Clone B-F34 Azide Free Capture mAb

This antibody can be used as a Capture Antibody in a human p53 sandwich immunoassay to detect human p53 in combination with biotinylated human p53 Detection Antibody (Cat No CDM437).

Background: p53 is a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons (PMIDs: 12032546, 20937277).

Entrez Symbol:

TP53

Data PDF:

CDM413

Specificity:

Recognizes wild type and mutant p53, the epitope is within residues 18-30 of human p53

Host Species:

Mouse

Immunogen:

Recombinant p53 protein

Isotype:

IgG2B

Clone:

B-F34

Conjugate:

Unconjugated

Formulation:

Liquid in PBS. Sterile-filtered through 0.22 µm. Carrier and Preservative free.

Purification:

Ion exchange chromatography

Application:

ELISA

Application notes:

ELISA Capture Antibody: 1 - 5 µg/ml

Storage/Stability:

Store at 2-8°C for 12 months. For longer storage, freeze aliquots at -20°C. Avoid repeated freeze-thaw cycles.