Mouse Anti-Human TNF-alpha Clone B-F7 Azide Free Capture mAb

Molecule:

TNF-alpha

Synonyms:

DIF, TNF-alpha, TNFA, TNFSF2

Species:

Human

Cat. No.:

CDM412 pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$995.00 BUY

Concentration:

1 mg/ml

Description:

Mouse Anti-Human TNF-alpha Clone B-F7 Azide Free Capture mAb

This antibody can be used as a Capture Antibody in a human TNF-alpha sandwich immunoassay to detect human TNF-alpha in combination with biotinylated human TNF-a Detection Antibody (Cat No CDM436).

Background: TNF-alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily and is mainly secreted by macrophages. It can bind to and functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF-alpha has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

Entrez Symbol:

TNF

Uniprot Name:

Tumor necrosis factor

Uniprot ID:

P01375

Data PDF:

CDM412

Specificity:

Human | Recognizes both natural and recombinant TNF-alpha

Host Species:

Mouse

Immunogen:

Recombinant human TNF-alpha

Isotype:

IgG1

Clone:

B-F7

Conjugate:

Unconjugated

Formulation:

Liquid in phosphate-buffered saline. Sterile-filtered through 0.22 μm. Carrier and Preservative free.

Purification:

Ion exchange chromatography

Application:

ELISA

Application notes:

Capture Antibody:
ELISA: 1 - 10 µg/ml
ELISPOT: 5 - 10 µg/ml

Storage/Stability:

Store at 2-8°C for 12 months. For longer storage, freeze aliquots at -20°C. Avoid repeated freeze-thaw cycles.