Mouse IgG Easy Quantification ELISA Kit

Molecule:

IgG

Species:

Mouse

Cat. No.:

IS003A pdf (datasheet)

Quantity/Size:

1 x 96 tests

Price:

$495.00 BUY

Description:

The Mouse IgG Easy Quantification Kit provides a rapid and easy method (one antibody step ELISA) for the quantitative determination of mouse IgG in cell culture supernatant and ascitic fluid. The kit includes ready-to-use reagents necessary to analyze up to 90 samples in 30 min. Buffer solutions are color coded in order to simplify pipetting steps.

Principle of the Assay:
The method employs the quantitative sandwich enzyme immunoassay technique. A polyclonal antibody specific to mouse IgG (H+L) is pre-coated onto the microwells. Samples and standards are pipetted into microwells, and mouse IgG present in the sample is bound by the capture antibody. Next, a HRP (horseradish peroxidase) conjugated anti-mouse IgG (H+L) antibody is pipetted and incubated simultaneously with samples. After washing the microwells in order to remove any non-specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops proportionally to the amount of mouse IgG in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.

Specificity:
The method enables the detection of all IgG (IgG3 quantification requires a specific standard curve). Cross reactions (determined by ELISA) are < 1% for Human IgG, < 1.5% for Cow IgG, < 2% for Goat IgG, < 5 % for Swine IgG and <15 % for Guinea Pig and Rat IgG. The cross reaction with human serum and fetal calf serum is typically below 0.2%.

Sensitivity:
The detection range is from 20 ng/ml to 1900 ng/ml.

Data PDF:

IS003

Storage/Stability:

All kit components are stable for 12 months when stored at 2-8°C. Do not freeze.

Application:

ELISA