Mouse Immunoglobulin Easy Isotyping ELISA Kit

Molecule:

IgG

Species:

Mouse

Cat. No.:

IS001B pdf (datasheet)

Quantity/Size:

5 x 12 tests

Price:

$575.00 BUY

Description:

The Mouse Immunoglobulin Easy Isotyping Kit provides a rapid and easy method (2-step ELISA) to characterize mouse monoclonal antibody isotypes in cell culture supernatants or purified antibodies preparations. The kit includes ready-to-use reagents necessary to analyze 12 samples in less than 30 minutes. Buffer solutions are color coded in order to simplify pipetting steps. The method employs the quantitative sandwich enzyme immunoassay technique. Anti-mouse antibodies specific to each of the common light and heavy chains are pre-coated in the wells. Samples are pipetted into microwells and Ig present in the sample are bound by the capture antibody. Then, a HRP (horseradish peroxidase) conjugated anti-mouse IgG (H+L)antibody is pipetted and incubated simultaneously with samples. After washing microwells in order to remove any non specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops if the specific immunoglobulin is present in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.

Principle of the Assay:
The method employs the quantitative sandwich enzyme immunoassay technique. Anti-mouse antibodies specific to each of the common light and heavy chains are pre-coated in the wells. Samples are pipetted into microwells and Ig present in the sample is bound by the capture antibody. Then, a HRP (horseradish peroxidase) conjugated anti-mouse IgG (H+L) antibody is pipetted and incubated simultaneously with samples. After washing microwells in order to remove any non-specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops if the specific immunoglobulin is present in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.

Specificity:
The method enables the detection of Light chains (Kappa and Lambda), and Heavy chains (Gamma 1, Gamma 2a, Gamma 2b, Gamma 3, and Mu) of mouse immunoglobulin.

Data PDF:

IS001

Kit Plates:

12 strips of 8 microwells

Storage/Stability:

All kit components are stable for 12 months when stored at 2-8°C. Do not freeze. After opening, store reagents at 2-8°C, handle with care to avoid contamination and use within 2 months.

Application:

ELISA