Streptavidin

Molecule:

Streptavidin

Cat. No.:

CSI12859 pdf (datasheet)

Quantity/Size:

100 mg

Price:

$1,195.00 BUY

Description:

Background: Streptavidin is a tetrameric protein secreted by Streptomyces avidinii which binds firmly to biotin. Streptavidin is wilyde used in molecular biology through its unique high affinity for the vitamin biotin. The dissociation constant (Kd) of th

Uniprot Name:

Streptavidin

Uniprot ID:

P22629

Data PDF:

CSI12857

Source:

Bacterium Streptomyces avidinii

Molecular Weight:

53 kDa

Physical State:

Lyophilized

Formulation:

Lyophilized from a sterile filtered solution in 10 mM potassium phosphate buffer, pH 6.5.

Purity:

>95% by SDS-PAGE analyses.

Biological Activity:

<10-3 U/mg protein (Azocoll, 25�C, 24 h, pH 8.0).

Specific Activity:

>15.3 U/mg (1 unit binds 1 �g biotin).

Endotoxin Level:

<0.1 ng/�g

Reconstitution:

Centrifuge vial prior to opening. Reconstitute in 0.1 M NaCl to a concentration of 5 mg/ml. The solution can then be further diluted in other aqueous buffers.

Storage/Stability:

Lyophilized protein is stable at 2-4℃C for 3 weeks, but is preferably stored dessicated at -20℃C. For long term storage, aliquot and store at -20℃C in dissolved form with 1 mM EDTA and/or 0.02 % NaN3 or pass the solution through a steril