Protein A Easy ELISA Kit

Cat. No.:

IS006 pdf (datasheet)

Quantity/Size:

1 x 96 tests

Price:

$495.00 BUY

Description:

The Protein A ELISA kit provides a quick and simple method to estimate the contamination by protein A from Staphylococcus aureus in a solution of antibodies. Ready-to-use reagents are sufficient for the analysis of 89 samples in 80 min. The kit includes colored buffers to facilitate and control distribution of samples in wells.

Principle of the Assay:Capture antibodies coated on the wells bind the protein A present in the sample and form complexes that are revealed by an anti-Protein A peroxidase conjugated detector antibody. After washing to remove any non-specific binding, the ready-to-use substrate solution is added to microwells and color develops proportionally to the amount of protein A in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm

Specificity: Cross reactions: no cross reaction observed with antibodies Matrix effect: the sample buffer, in general, has no effect on the assay. It is nevertheless recommended to test the effect of the buffer. Hook Effect: no hook effect observed.

Sensitivity: The detection range is from 64 pg/ml to 4,000 pg/ml.

Data PDF:

IS006

Storage/Stability:

All kit components are stable for 12 months when stored at 2-8°C. Do not freeze.

Application:

ELISA