Rabbit Anti-Human GNAI2 pAb

Molecule:

GNAI2

Synonyms:

GNAI2, G protein subunit alpha i2, GIP, GNAI2B, H_LUCA15.1, H_LUCA16.1

Species:

Human

Cat. No.:

CPG201 pdf (datasheet)

Quantity/Size:

200 μg

Price:

$280.00 BUY

Description:

Rabbit Anti-Human Galpha-i2 Polyclonal Antibody
Background: Gαi2 is a 40-41 kDa alpha-subunit of heterotrimeric protein, that shares sequence homology with Gαi3 and Gαi1. All these G proteins can be ADP-ribosylated at the carboxy terminal Cys of the CAAX sequence, interfering with receptor coupling. Gαi2 is expressed widely in tissues and cell lines. Activation of PLC-b by Gaicoupled receptors is the result of release of Gbg subunits from Gαi. Gαi2 regulates potassium channel activation. Mutation at Arg179 has been identified in tumors. This and the Q205L mutant inhibit adenylyl cyclase.

Entrez Symbol:

GNAI2

Uniprot Name:

GNAI2_HUMAN

Uniprot ID:

P04899

Data PDF:

CPG201

Specificity:

Recognizes both natural and recombinant Human Gαi2

Host Species:

Rabbit

Immunogen:

E. Coli expressed human Gαi2 (a.a. 231-351)

Isotype:

IgG

Conjugate:

Unconjugated

Formulation:

Lyphilized from 1xPBS

Purification:

Protein A Chromatography

Reconstitution:

Centrifuge vial prior to opening. Add 200 μl of sterile, distilled water to the vial to fully solubilize the antibody to a concentration of 1 mg/ml.

Application:

WB

Application notes:

Western blot: working dilution of 1:5,000

Storage/Stability:

Lyophilized product is stable at room temperature for shipping purposes. Upon receipt, store desiccated at at -20°C for up to 1 year. After reconstitution, the preparation is stable for up to one month at 2-8°C. For long term storage, freeze in working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles.