Rabbit Anti-Rat MCP-1/CCL2 (aa 1-73) pAb

Molecule:

MCP-1

Synonyms:

MCP-1, Scya2, Sigje, monocyte chemoattractant protein-1, monocyte chemotactic protein 1, small inducible cytokine A2, small inducible gene JE, Ccl2, C-C motif chemokine ligand 2

Species:

Rat

Cat. No.:

CPM001 pdf (datasheet)

Quantity/Size:

0.5 mg

Price:

$280.00 BUY

Description:

Rabbit Anti-Rat MCP-1 aa 1-73 polyclonal antibody.
Background: Rat MCP1 is a 148 amino acid CC chemokine with a NH2-terminal sequence of 29 residues as a signal sequence. It was originally cloned from Con A-stimulated rat spleen cDNA library. This rat MCP1 is 49 amino acids longer than human MCP1 at 3' end. This 3' end is a serine and threonine rich zone, which is probably responsible for the extensive O-glycosylation and which explains the higher molecular weight (25 kDa). In vitro, MCP1 is chemotactic for monocytes as well as lymphocytes and basophils, but not for neutrophils. MCP1 is produced by a wide range of cell types as a reaction to diverse inflammatory stimuli.

Entrez Symbol:

Ccl2

Uniprot Name:

CCL2_RAT

Uniprot ID:

P14844

Data PDF:

CPM001

Specificity:

Recognizes both natural and recombinant rat MCP-1/CCL2

Host Species:

Rabbit

Immunogen:

Recombinant N-terminal of Rat MCP-1 (aa 1-73)

Isotype:

IgG

Conjugate:

Unconjugated

Formulation:

Lyphilized from 1xPBS

Purification:

Protein A Chromatography

Reconstitution:

Centrifuge vial prior to opening. Reconstitute to 1 mg/ml by adding 0.5 ml H2O.

Application:

IHC, NT, WB

Application notes:

Western blot: working dilution of 1:2000

Storage/Stability:

Lyophilized product is stable at room temperature for shipping purposes. Upon receipt, store desiccated at at -20°C for up to 1 year. After reconstitution, the preparation is stable for up to one month at 2-8°C. For long term storage, freeze in working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles.