Rabbit Anti-Rat Perforin 1 Neutralizing pAb

Molecule:

Perforin-1

Synonyms:

PRF1, Cyta, RATCYTA, cytolysin, lymphocyte pore-forming protein

Species:

Rat

Cat. No.:

CPP100 pdf (datasheet)

Quantity/Size:

200 μg

Price:

$280.00 BUY

Description:

Rabbit Anti-Rat Perforin 1 Neutralizing pAb
Background: Perforin (PRF1) was originally cloned from Mouse cytotoxic T-lymphocyte (CTL). It is a 555-amino acid protein with a 21-amino acid signal peptide. PRF1 is a cytolytic mediator produced by killer lymphocytes, and is stored in and released by cytoplasmic granules. It is involved in the killing mediated by CTL which represents an important mechanism in the immune defense against tumors and virus infections.

Entrez Symbol:

Prf1

Uniprot Name:

Q5FVS5_RAT

Uniprot ID:

Q5FVS5

Data PDF:

CPP100

Specificity:

Recognizes both natural and recombinant rat perforin

Host Species:

Rabbit

Immunogen:

Recombinant rat perforin from E. coli, aa 21-534 (full length mature)

Isotype:

IgG

Conjugate:

Unconjugated

Formulation:

Lyphilized from 1xPBS

Purification:

Protein A Chromatography

Reconstitution:

Centrifuge vial prior to opening. Add 200 μl of sterile, distilled water to the vial to fully solubilize the antibody to a concentration of 1 mg/ml.

Cross Reactivity:

1255

Application:

IHC, WB

Application notes:

Western blot: working dilution of 1:1000
Immunohistochemistry: working dilution of 1:100-1:300.

Storage/Stability:

Lyophilized product is stable at room temperature for shipping purposes. Upon receipt, store desiccated at at -20°C for up to 1 year. After reconstitution, the preparation is stable for up to one month at 2-8°C. For long term storage, freeze in working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles.