Rat IgG Easy Quantification ELISA Kit

Molecule:

IgG

Species:

Rat

Cat. No.:

IS004B pdf (datasheet)

Quantity/Size:

10 x 96 tests

Price:

$2,425.00 BUY

Description:

The Rat IgG Easy Quantification ELISA Kit provides a rapid and easy method (one antibody step ELISA) for the quantitative determination of rat IgG in cell culture supernatant and rat ascitic fluid. The kit includes ready-to-use reagents necessary to analyze up to 89 samples in 30 min. Buffer solutions are color coded in order to simplify pipetting steps.

Principle of the Assay:
The method employs the quantitative sandwich enzyme immunoassay technique. A polyclonal antibody specific to rat IgG (H+L) is pre-coated onto the microwells. Samples and standards are pipetted into microwells, and rat IgG present in the sample is bound by the capture antibody. Then, a HRP (horseradish peroxidase) conjugated anti-rat IgG (H+L) antibody is pipetted and incubated simultaneously with samples. After washing the microwells in order to remove any non-specific binding, the ready to use substrate solution (TMB) is added to microwells. Color develops proportionally to the amount of rat IgG in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.

Specificity:
Detection of all rat subclasses of IgG No cross reaction was observed by ELISA with Human and bovine IgG. Cross reactions with mouse IgG are < 8%.

Sensitivity:
The detection range is from 16 ng/ml to 2000 ng/ml. The detection threshold is 4 ng/ml.

Data PDF:

IS004

Storage/Stability:

All kit components are stable for 12 months when stored at 2-8°C. Do not freeze.

Application:

ELISA