Recombinant Bovine FGF2/FGF-basic

Molecule:

FGF2/FGF-basic

Synonyms:

BFGF, FGFB, HBGF-2, basic fibroblast growth factor, bFGF, fibroblast growth factor 2, heparin-binding growth factor 2

Species:

Bovine

Cat. No.:

CRF023A pdf (datasheet)

Quantity/Size:

10 μg

Price:

$165.00 BUY

Description:

Recombinant Bovine Fibroblast Growth Factor-basic is a single non-glycosylated polypeptide chain containing 147 amino acids.

Background: Fibroblast Growth Factor-basic (bFGF) is a single-chain polypeptide growth factor that plays a significant role in the process of wound healing and is a potent inducer of angiogenesis. Several different forms of the human protein exist ranging from 18-24 kDa in size due to the use of alternative start sites within the fgf-2 gene. It has a 55 percent amino acid residue identity to FGF-1 and has potent heparin-binding activity. The growth factor is an extremely potent inducer of DNA synthesis in a variety of cell types from mesoderm and neuroectoderm lineages. It was originally named basic fibroblast growth factor based upon its chemical properties and to distinguish it from acidic fibroblast growth factor.Other homologous FGF belonging to the same family are int-2 ( FGF-3 ), FGF-5 , FGF-6 , K-FGF and KGF ( keratinocyte growth factor, FGF-7 ). All factors are products of different genes, some of which are Oncogene products (FGF-3, FGF-4, FGF-5).

Entrez Symbol:

FGF2

Uniprot Name:

Heparin-binding growth factor 2

Uniprot ID:

P03969

Data PDF:

CRF023

Source:

E. coli

Molecular Weight:

~16.5 kDa

Physical State:

Lyophilized

Formulation:

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

Purity:

>97% by SDS-PAGE and HPLC analyses.

Biological Activity:

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using mouse balb/c 3T3 cells is less than 0.1 ng/ml.

Endotoxin Level:

Less than 1EU/μg as determined by LAL method.

Amino Acid Sequence:

MPALPEDGGS GAFPPGHFKD PKRLYCKNGG FFLRIHPDGR VDGVREKSDP HIKLQLQAEE RGVVSIKGVC ANRYLAMKED GRLLASKCVT DECFFFERLE SNNYNTYRSR KYSSWYVALK RTGQYKLGPK TGPGQKAILF LPMSAKS

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/mL. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

Stable at 2-8°C, but best kept desiccated -20°C. Upon reconstitution, stable for up to 1 week at 2-8°C. For longer term, store in working aliquots below -20°C. Avoid repeated freeze/thaw cycles.