Recombinant E. coli Methionine Aminopeptidase His

Synonyms:

pepM

Species:

E. coli

Cat. No.:

CS427B pdf (datasheet)

Quantity/Size:

20 μg

Price:

$300.00 BUY

Description:

Methionine aminopeptidases and designated peptidase M proteins belong to the M24 family of proteins. MAP protein removes the amino-terminal methionine residue from nascent polypeptides. The active site of MAP contains 2 adjacent divalent metal ions connected by a water molecule or hydroxide ion. MAP produced in E. coli is a single, non-glycosylated polypeptide chain containing 284 amino acids (1-264 aa). The protein is fused to a 20 amino acid His-tag at the N-terminus and purified by proprietary chromatographic techniques.

Entrez Symbol:

map

Data PDF:

CS427

Source:

E. coli

Molecular Weight:

31.5 kDa

Tag:

His-tagged

Physical State:

Liquid

Formulation:

Sterile Filtered clear solution of protein (1 mg/ml) in 20 mM Tris-HCl buffer, pH 8.0, + 10% glycerol + 2 mM DTT.

Purity:

>90% as determined by SDS-PAGE.

Amino Acid Sequence:

MGSSHHHHHH SSGLVPRGSH MAISIKTPED IEKMRVAGRL AAEVLEMIEP YVKPGVSTGE LDRICNDYIV NEQHAVSACL GYHGYPKSVC ISINEVVCHG IPDDAKLLKD GDIVNIDVTV IKDGFHGDTS KMFIVGKPTI MGERLCRITQ ESLYLALRMV KPGINLREIG AAIQKFVEAE GFSVVREYCG HGIGRGFHEE PQVLHYDSRE TNVVLKPGMT FTIEPMVNAG KK

Storage/Stability:

Store at 2-4οΨΑC if entire vial will be used within 2-4 weeks. Store frozen in aliquots at -20οΨΑC to -80οΨΑC for longer periods of time. For long term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cy