Recombinant Human Adiponectin/Acrp30

Synonyms:

ACDC; ADPN; APM1; APM-1; GBP28; ACRP30; ADIPQTL1

Species:

Human

Cat. No.:

CRA025B pdf (datasheet)

Quantity/Size:

25 μg

Price:

$245.00 BUY

Description:

Globular ACRP-30, gACRP-30, is a naturally occurring cleavage product of adiponectin, a molecule made exclusively by adipocytes. gACRP-30 is detected a relatively high concentrations in the serum and is thought to play an important role in hyperglycemia and insulin resistance. gACRP-30 signals through receptors, AdipoR1 and AdipoR2. Recombinant human gACRP-30 is a non-glycosylated protein, containing145 amino acids, with a molecular weight of 16.7 kDa.

Entrez Symbol:

ADIPOQ

Data PDF:

CRA025

Source:

E. coli

Molecular Weight:

16.7 kDa

Formulation:

Recombinant human gACRP-30 is lyophilized with 10 mM Tris, pH 8.0 and 0.75 mM DTT at a concentration of 1 mg/mL.

Purity:

> 95% as determined by Reducing and Non-reducing SDS-PAGE, UV spectroscopy at 280 nm

Biological Activity:

The activity is determined by the ability to inhibit the proliferation of mouse M1 cells and is typically 1.0 – 2.5 μg/mL.

Endotoxin Level:

Measured by kinetic LAL analysis and is typically ≤ 1 EU/μg protein.

Amino Acid Sequence:

MKGEPGEGAY VYRSAFSVGL ETYVTIPNMP IRFTKIFYNQ QNHYDGSTGK FHCNIPGLYY FAYHITVYMK DVKVSLFKKD KAMLFTYDQY QENNVDQASG SVLLHLEVGD QVWLQVYGEG ERNGLYADND NDSTFTGFLL YHDTN

Reconstitution:

Centrifuge vial prior to opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile 5 mM Tris, pH

Storage/Stability:

Store lyophilized protein at -20°C. Reconstituted protein is stable for 2 weeks at 2-4°C. For long term storage, aliquot and store at -20°C. Avoid repeated freeze-thaw cycles.