Recombinant Human Artemin

Synonyms:

ART; EVN; NBN; ENOVIN

Species:

Human

Cat. No.:

CRA001A pdf (datasheet)

Quantity/Size:

5 μg

Price:

$135.00 BUY

Description:

Artemin is a novel member of the glial cell line-derived neurotrophic factor (GNDF) ligand family. Current evidence suggests that Artemin signals through the receptor complex, GFRalpha3-RET, to influence neuron survival in vitro and in vivo.

Recombinant human Artemin is a non-glycosylated, disulfide-linked homodimer, containing two 113 amino acid chains.

Entrez Symbol:

ARTN

Data PDF:

CRA001

Source:

E. coli

Molecular Weight:

24.2 kDa

Formulation:

Recombinant Artemin is lyophilized with 10 mM sodium citrate pH 4.5 and 25 mM NaCl.

Purity:

≥98.0% as determined by Reducing and Non-reducing SDS-PAGE, HPLC

Biological Activity:

The activity is determined by the dose-dependant proliferation of the SH-SY5Y cell line and is typically 4-8 ng/mL. The activity can also be determined by its ability to promote survival and neurite outgrowth.

Endotoxin Level:

Measured by kinetic LAL analysis and is typically ≤ 1 EU/μg protein.

Amino Acid Sequence:

AGGPGSRARA AGARGCRLRS QLVPVRALGL GHRSDELVRF RFCSGSCRRA RSPHDLSLAS LLGAGALRPP PGSRPVSQPC CRPTRYEAVS FMDVNSTWRT VDRLSATACG CLG

Reconstitution:

Centrifuge vial prior to opening. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/mL, which can be further diluted into other aqueous solutions.

Storage/Stability:

Lyophilized product is very stable at -20°C. Reconstituted material should be aliquoted and frozen at -20°C. It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage.