Recombinant Human Artemin

Synonyms:

ART; EVN; NBN; ENOVIN

Species:

Human

Cat. No.:

CRA001C pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$2,700.00 BUY

Description:

Artemin is a novel member of the glial cell line-derived neurotrophic factor (GNDF) ligand family. Current evidence suggests that Artemin signals through the receptor complex, GFRalpha3-RET, to influence neuron survival in vitro and in vivo. Recombinant human Artemin is a non-glycosylated, disulfide-linked homodimer, containing two 113 amino acid chains.

Entrez Symbol:

ARTN

Data PDF:

CRA001

Source:

E. coli

Molecular Weight:

24.2 kDa

Formulation:

Recombinant Artemin is lyophilized with 10 mM sodium citrate pH 4.5 and 25 mM NaCl.

Purity:

≥98.0% as determined by Reducing and Non-reducing SDS-PAGE, HPLC

Biological Activity:

The activity is determined by the dose-dependant proliferation of the SH-SY5Y cell line and is typically 4-8 ng/mL. The activity can also be determined by its ability to promote survival and neurite outgrowth.

Endotoxin Level:

Measured by kinetic LAL analysis and is typically ≤ 1 EU/μg protein.

Amino Acid Sequence:

AGGPGSRARA AGARGCRLRS QLVPVRALGL GHRSDELVRF RFCSGSCRRA RSPHDLSLAS LLGAGALRPP PGSRPVSQPC CRPTRYEAVS FMDVNSTWRT VDRLSATACG CLG

Reconstitution:

Centrifuge vial prior to opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a c

Storage/Stability:

Lyophilized product is very stable at -20°C. Reconstituted material should be aliquoted and frozen at -20°C. It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage.