Recombinant Human Beta Defensin 4

Molecule:

BD-4

Synonyms:

beta Defensin 4; defensin beta 104A; BD4; BD-4; DEFB4; hBD-4; DEFB-4; DEFB104

Species:

Human

Cat. No.:

CRB135C pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$2,700.00 / Special Price: $1,640.00 BUY

Description:

Recombinant Human Beta Defensin 4 is a a single non-glycosylated polypeptide chain containing 50 amino acids. Background: Defensins (alpha and beta) are cationic peptides with antimicrobial activity against Gram-negative and Gram-positive bacteria, fungi, and enveloped viruses. They are 2-6 kDa proteins and take important roles in the innate immune system. On the basis of their size and pattern of disulfide bonding, mammalian defensins are classified into alpha, beta and theta categories. Beta-Defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. Four human beta-defensins have been identified and they are expressed on some leukocytes and at epithelial surfaces. Because beta-defensins is cationic peptides, they can therefore interact with the membrane of invading microbes, which are negative due to lipopolysaccharides (LPS) and lipoteichoic acid (LTA) found in the cell membrane. Especially, they have higher affinity to the binding site compared to Ca2+ and Mg2+ ions. Furthermore, they can affect the stability of the membrane.

Entrez Symbol:

DEFB104A

Uniprot Name:

Beta-defensin 104

Uniprot ID:

Q8WTQ1

Data PDF:

CRB135

Source:

E. coli

Molecular Weight:

~6.0 kDa

Formulation:

Lyophilized from a 0.2μm filtered concentrated solution in 20 mM PB, pH 7.4 + 130 mM NaCl.

Purity:

>98% by SDS-PAGE and HPLC analyses.

Biological Activity:

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 0.1-100.0 ng/ml.

Amino Acid Sequence:

EFELDRICGY GTARCRKKCR SQEYRIGRCP NTYACCLRKW DESLLNRTKP

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

This lyophilized preparation is stable at 2-4°C, but should be kept desiccated at -20°C for long term storage. Upon reconstitution, the preparation is stable for up to one week at 2-4°C.