Recombinant Human BDNF

Molecule:

BDNF

Synonyms:

Abrineurin, BDNF, ANON2, BULN2

Species:

Human

Cat. No.:

CRB600B pdf (datasheet)

Quantity/Size:

10 μg

Price:

$235.00 BUY

Description:

Recombinant human BDNF is a non-glycosylated homodimer, containing two 119 amino acid chains, with a total molecular weight of 27 kDa.
Background: Brain Derived Neurotrophic Factor, or BDNF, is a nerve growth factor that supports neuron growth and survival. BDNF shares identical domains with two other neurotrophic factors known as, β-NGF and NT-3 (neurotrophin-3). BDNF binds with low affinity to a receptor known as LNGFR, which also binds NGF and NT-3, but mediates survival function by signaling through a high affinity receptor known as gp145/TrkB. Human, mouse, rat and pig BDNF are all cross-reactive.

Entrez Symbol:

BDNF

Uniprot Name:

Brain-derived neurotrophic factor

Data PDF:

CRB600

Genbank Accession No:

P23560

Source:

E. coli

Molecular Weight:

~27 kDa

Physical State:

Lyophilized

Formulation:

Lyophilized from a sterile filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA).

Purity:

≥95.0% by SDS-PAGE

Biological Activity:

The ED50 as determined by the dose-dependent proliferation of C6 cells is typically between 0.5-1.5 μg/mL

Specific Activity:

1 x 10E3 units/mg

Endotoxin Level:

≤1 U/µg of protein by kinetic LAL analysis (50% confidence range).

Amino Acid Sequence:

MHSDPARRGE LSVCDSISEW VTAADKKTAV DMSGGTVTVL EKVPVSKGQL KQYFYETKCN PMGYTKEGCR GIDKRHWNSQ CRTTQSYVRA LTMDSKKRIG WRFIRIDTSC VCTLTIKRGR

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water to a concentration of 0.1 mg/ml. DO NOT VORTEX. Allow several minutes for complete reconstitution. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

Lyophilized product is stable at room temperature for shipping purposes. Upon receipt, store desiccated at -20°C for up to 1 year. Upon reconstitution, the preparation is stable for up to one month at 2-8°C. For long term storage, freeze in working aliquots and store at -20 to -80°C. For maximal stability, dilute to working aliquots in a 0.1% BSA solution. Avoid repeated freeze-thaw cycles.