Recombinant Human Beta Defensin 1

Molecule:

BD-1

Synonyms:

BD1, DEFB-1, DEFB101, HBD1, beta-defensin-1

Species:

Human

Cat. No.:

CRB503C pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$2,700.00 / Special Price: $1,640.00 BUY

Description:

Recombinant Human BD-1 is a single non-glycosylated polypeptide chain containing 47 amino acids.

Background: Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The alpha-defensins are distinguished from the beta-defensins by the pairing of their three disulfide bonds. To date, four human beta-defensins have been identified; BD-1, BD-2, BD-3 and BD-4. Beta-defensins are expressed on some leukocytes and at epithelial surfaces. In addition to their direct antimicrobial activities, they are chemoattractant towards immature dendritic cells and memory T cells. The beta-defensin proteins are expressed as the C-terminal portion of precursors and are released by proteolytic cleavage of a signal sequence and, in the case of BD-1 (36 a.a.), a propeptide region. Beta-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. Beta-Defensins are 3-5 kDa peptides ranging in size from 33-47 amino acid residues.

Entrez Symbol:

DEFB1

Uniprot Name:

Beta-defensin 1

Uniprot ID:

P60022

Data PDF:

CRB503

Source:

E. coli

Molecular Weight:

~5.1 kDa

Formulation:

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4 + 130 mM NaCl.

Purity:

>98% by SDS-PAGE and HPLC analyses.

Biological Activity:

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using CD34+ dendritic cells is in a concentration range of 100.0-1000.0 ng/ml.

Endotoxin Level:

Less than 1EU/μg as determined by LAL method.

Amino Acid Sequence:

GNFLTGLGHR SDHYNCVSSG GQCLYSACPI FTKIQGTCYR GKAKCCK

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

This lyophilized preparation is stable at 2-4°C, but should be kept desiccated at -20°C for long term storage. Upon reconstitution, the preparation is stable for up to one week at 2-4°C.