Recombinant Human CXCL13/BLC/BCA-1

Molecule:

CXCL13

Synonyms:

B Cell-Attracting chemokine 1, BCA1, BLC, BLR1L, SCYB13, ANGIE, ANGIE2, B lymphocyte chemoattractant, CXC chemokine BLC, Small-inducible cytokine B13

Species:

Human

Cat. No.:

CRB400B pdf (datasheet)

Quantity/Size:

10 μg

Price:

$235.00 BUY

Description:

Recombinant Human BCA-1/CXCL13 is a single non-glycosylated polypeptide chain containing 87 amino acids.

Background: BCA-1/BLC/CXCL13, also known as B-lymphocyte chemoattractant (BLC), is a CXC chemokine that is constitutively expressed in secondary lymphoid organs. BCA-1 cDNA encodes a protein of 109 amino acid residues with a leader sequence of 22 residues. Mature human BCA-1 shares 64% amino acid sequence similarity with the mouse protein and 23 - 34% amino acid sequence identity with other known CXC chemokines. Recombinant or chemically synthesized BCA-1 is a potent chemoattractant for B lymphocytes but not T lymphocytes, monocytes or neutrophils. BLR1, a G protein-coupled receptor originally isolated from Burkitt’s lymphoma cells, has now been shown to be the specific receptor for BCA-1. Among cells of the hematopoietic lineages, the expression of BLR1, now designated CXCR5, is restricted to B lymphocytes and a subpopulation of T helper memory cells. Mice lacking BLR1 have been shown to lack inguinal lymph nodes. These mice were also found to have impaired development of Peyer’s patches and defective formation of primary follicles and germinal centers in the spleen as a result of the inability of B lymphocytes to migrate into B cell areas. The protein is a single, non-glycosylated polypeptide chain containing 87 amino acids.

Entrez Symbol:

CXCL13

Data PDF:

CRB400

Source:

E. coli

Molecular Weight:

~ 10.3 kDa

Formulation:

Lyophilized from a sterile filtered solution in 20mM PB, pH 7.4 + 100 mM NaCl.

Purity:

>97% by SDS-PAGE and HPLC analyses.

Biological Activity:

Fully biologically active compared to standard. The biological activity determined by a chemotaxis bioassay using human B cells is in a concentration range of 1.0-10 ng/ml.

Endotoxin Level:

Less than 1 EU/μg of rHuBCA-1/CXCL13 as determined by LAL method.

Amino Acid Sequence:

VLEVYYTSLR CRCVQESSVF IPRRFIDRIQ ILPRGNGCPR KEIIVWKKNK SIVCVDPQAE WIQRMMEVLR KRSSSTLPVP VFKRKIP

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/mL. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

Stable at 2-4°C, but best kept desiccated -20°C. Upon reconstitution, stable for up to 1 week at 2-8°C. For longer term, divide into working aliquots and store at -20°C to -80°C