Recombinant Human BMP-4 inactive

Molecule:

BMP-4

Synonyms:

Bone Morphogenetic Protein 4, ZYME, BMP2B, BMP2B1

Species:

Human

Cat. No.:

CRB105C pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$1,900.00 / Special Price: $1,640.00 BUY

Description:

Recombinant Human Bone Morphogenetic Protein-4 is a monomeric, non-glycosylated, polypeptide chain containing 117 amino acids. This item is not biologically active. If you need a biologically active form of Recombinant Human BMP4, see Catalog Nos. CRB137A, CRB137B & CRB137C.

Applications:

1. Molecular standard (Western, ELISA) in studying secreted BMP-4

2. Preparing antibodies for BMP-4 monomer

3. Molecule standard in detecting secreted BMP-4 in reduced SDS-PAGE

Background: Bone Morphogenetic Protein 4 is one of the BMPs, some of which belong to the TGF-beta superfamily (BMP2-7). There are more than thirteen BMPs have been discovered nowadays and they are involved in inducing cartilage and bone formation. BMP-4 is expressed in the lung and lower levels seen in the kidney.  It also presents in normal and neoplastic prostate tissues, and prostate cancer cell lines. It regulates the formation of teeth, limbs and one from mesoderm.  Furthermore it also plays a role in fracture repair.  BMP-4 signals through tetrameric complexes composed of type I and type II receptors and regulates it function by interaction with multiple proteins and glycosaminoglycans. The human BMP-4 shares 98 % sequence indentity with mouse BMP-4.  Reduced expression of BMP-4 is associated with a number of bone diseases, including the heritable disorder Fibrodysplasia Ossificans Progressiva.

Entrez Symbol:

BMP4

Uniprot Name:

Bone morphogenetic protein 4

Uniprot ID:

P12644

Data PDF:

CRB105

Source:

E. coli

Molecular Weight:

~13.3 kDa

Physical State:

Lyophilized

Formulation:

Lyophilized from a 0.2 µm filtered concentrated solution in 50 mM Na2CO3, 5 mM DTT, pH 11.0.

Purity:

>95% by SDS-PAGE and HPLC analyses

Biological Activity:

Data is not available.

Endotoxin Level:

Less than 1 EU/µg of recombinant Human BMP-4 as determined by LAL method.

Amino Acid Sequence:

MSPKHHSQRA RKKNKNCRRH SLYVDFSDVG WNDWIVAPPG YQAFYCHGDC PFPLADHLNS TNHAIVQTLV NSVNSSIPKA CCVPTELSAI SMLYLDEYDK VVLKNYQEMV VEGCGCR

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

This lyophilized preparation is stable at 2-4°C, but should be kept desiccated at -20°C for long term storage. Upon reconstitution, the preparation is stable for up to one week at 2-4°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -80°C. Avoid repeated freeze/thaw cycles.

Application notes:

Molecular standard (Western, ELISA) in studying secreted BMP-4; Preparing antibodies for BMP-4 monomer; Molecule standard in detecting secreted BMP-4 in reduced SDS-PAGE .