Recombinant Human BMP-7, inactive

Molecule:

BMP-7

Synonyms:

Osteogenic Protein 1, OP-1

Species:

Human

Cat. No.:

CRB104C pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$3,600.00 / Special Price: $1,690.00 BUY

Description:

Recombinant Human BMP-7 is a monomeric, non-glycosylated polypeptide chain containing 139 amino acids.

Background: Bone Morphogenetic Protein 7 is one of the BMPs, some of which belong to the TGF-beta superfamily (BMP2-7). There are more than thirteen BMPs have been discovered nowadays and they are involved in inducing cartilage and bone formation. BMP-7 is mainly expressed in kidney and bladder. It is also present in developing eyes, brain and ear during embryogenesis. BMP-7 also named osteogenic protein-1 (OP-1) is a potent osteoinductive cytokine and plays role in osteoblast differentiation, SMAD1 production and renal development and repair. Human BMP-7 is synthesized with a signal sequence (29 a.a.), a propeptide (263 a.a.), and a growth factor domain (139 a.a.). The growth factor domain of human BMP-7 shares 98 % a.a. sequence identity with mouse and rat BMP-7.

Entrez Symbol:

BMP7

Data PDF:

CRB104

Source:

E. coli

Molecular Weight:

~15.7 kDa

Formulation:

Lyophilized from a 0.2 μm filtered concentrated solution in 30 % acetonitrile, 0.1 % TFA.

Purity:

>95% by SDS-PAGE and HPLC analyses

Biological Activity:

Data is not available.

Amino Acid Sequence:

STGSKQRSQN RSKTPKNQEA LRMANVAENS SSDQRQACKK HELYVSFRDLGWQDWIIAPE GYAAYYCEGE CAFPLNSYMN ATNHAIVQTL VHFINPETVP KPCCAPTQLNAISVLYFDDS SNVILKKYRN MVVRACGCH

Reconstitution:

Centrifuge vial prior to opening. Reconstitute in 10 mM HAc to a concentration of 0.1-1.0 mg/mL. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

This lyophilized preparation is stable at 2-4°C, but should be kept desiccated at -20°C for long term storage. Upon reconstitution, the preparation is stable for up to one week at 2-4°C.

Application notes:

1. Molecular standard (Western Blot, ELISA) in studying secreted BMP-7
2. Preparing antibodies for BMP-7 monomer.
3. Molecular standard in detecting secreted BMP-7 in reduced SDS-PAGE.

Application:

ELISA