Recombinant Human CAMK2A His tagged

Molecule:

CAMK2A

Synonyms:

CAMKA, CaMKIINalpha, CaMK-II alpha subunit, caMK-II subunit alpha, CaM-kinase II alpha chain, CaM kinase II alpha subunit, caM kinase II subunit alpha, calcium, calmodulin-dependent protein kinase II alpha-B subunit, calcium, calmodulin-dependent protein kinase typ

Species:

Human

Cat. No.:

CSI13335 pdf (datasheet)

Quantity/Size:

10 μg

Price:

$615.00 BUY

Description:

Product: Recombinant Human Full-Length Calmodulin-dependent Kinase IIa, histidine-tagged expressed in insect cells. No special measures were taken to activate this kinase. Mass Spectrometry: The enzyme preparation was subjected to tryptic digestion follow

Concentration:

0.34 mg/ml (Total protein as measured using the Bradford protein assay with BSA as a standard).

Entrez Symbol:

CAMK2A

Uniprot Name:

Calcium/calmodulin-dependent protein kinase type II subunit alpha

Uniprot ID:

Q9UQM7

EC number:

2.7.11.17

Data PDF:

CSI13335

Genbank Accession No:

NP_741960

Source:

Insect cells

Molecular Weight:

56.8 kDa

Tag:

His

Physical State:

Frozen liquid

Formulation:

Sterile filtered liquid (Frozen) in 20 mM Tris, pH 7.5 + 100 mM NaCl + 0.5 mM EDTA + 0.05% NP-40 + 2 mM DTT + 50% Glycerol.

Purity:

>90% by SDS-PAGE analysis.

Specific Activity:

7300 nmol/min/mg (phosphate transferred to autocamtide-2 per minute per mg of total protein at 30�C. Activity determined at a final protein concentration of 0.05 �g/ml).

Amino Acid Sequence:

MATITCTRFTEEYQLFEELGKGAFSVVRRCVKVLAGQEYAAKIINTKKLSARDHQKLEREARICRLLKHPNIVRLHDSISEEGHHYLIFDLVTGGELFEDIVAREYYSEADASHCIQQILEAVLHCHQMGVVHRDLKPENLLLASKLKGAAVKLADFGLAIEVEGEQQAWFGFAGTPGYLSPEVLRKDPYGKPVDLWACGVILYILLVGYPPFWDEDQHRLYQQIKAGAYDFPSPEWDTVTPEAKDLINKMLTIN

Storage/Stability:

Frozen liquid protein is stable at -80℃C for 6 months from date of purchase. For long term storage, aliquot and store at -80℃C. Avoid repeated freeze-thaw cycles.

Application notes:

The dilution buffer described is recommended for the radiometric assay format. For high throughput applications such as PolarScreen TM, Z'-LYTE TM, and LanthaScreen TM, modifications to this buffer are needed.