Recombinant Human CAMK2B His

Molecule:

CAMK2B

Synonyms:

CAM2, CAMK2, CAMKB, caMK-II subunit beta, CaM-kinase II beta chain, CaM kinase II beta subunit, caM kinase II subunit beta, proline rich calmodulin-dependent protein kinase, calcium, calmodulin-dependent protein kinase type II beta chain

Species:

Human

Cat. No.:

CSI13336 pdf (datasheet)

Quantity/Size:

10 μg

Price:

$615.00 BUY

Description:

Product: Recombinant Human Full-Length protein, Histidine-tagged, expressed in insect cells. No special measures were taken to activate this kinase. Mass Spectrometry: The enzyme preparation was subjected to tryptic digestion followed by LCMS/MS. The resu

Concentration:

0.44 mg/ml (Total protein as measured using the Bradford protein assay with BSA as a standard).

Entrez Symbol:

CAMK2B

Uniprot Name:

Calcium/calmodulin-dependent protein kinase type II subunit beta

Uniprot ID:

Q13554

EC number:

2.7.11.17

Data PDF:

CSI13336

Genbank Accession No:

NP_742078.1

Source:

Insect cells

Molecular Weight:

60.5 kDa

Tag:

His

Physical State:

Frozen liquid

Formulation:

Sterile filtered liquid (Frozen) in 50 mM Tris, pH 7.5 + 150 mM NaCl + 0.5 mM EDTA + 0.02% Triton X-100 +2 mM DTT + 50% Glycerol

Purity:

>90% by SDS-PAGE analysis.

Specific Activity:

774 nmol/min/mg (phosphate transferred to Autocamtide-2 peptide substrate (KKALRRQETVDAL) per minute per mg of total protein at 30�C. Activity determined at a final protein concentration of 1.67 �g/ml).

Amino Acid Sequence:

MSYYHHHHHHDYDIPTTENLYFQGITSLYKKAGTMATTVTCTRFTDEYQLYEDIGKGAFSVVRRCVKLCTGHEYAAKIINTKKLSARDHQKLEREARICRLLKHSNIVRLHDSISEEGFHYLVFDLVTGGELFEDIVAREYYSEADASHCIQQILEAVLHCHQMGVVHRDLKPENLLLASKCKGAAVKLADFGLAIEVQGDQQAWFGFAGTPGYLSPEVLRKEAYGKPVDIWACGVILYILLVGYPPFWDEDQHK

Storage/Stability:

Frozen liquid protein is stable at -80℃C for 6 months from date of purchase. For long term storage, aliquot and store at -80℃C. Avoid repeated freeze-thaw cycles.

Application notes:

The dilution buffer described is recommended for the radiometric assay format. For high throughput applications such as PolarScreen TM, Z'-LYTE TM, and LanthaScreen TM, modifications to this buffer are needed.