Recombinant Human CCL11/Eotaxin

Molecule:

Eotaxin

Synonyms:

SCYA11, eotaxin-1, C-C motif chemokine 11, small-inducible cytokine A11, eosinophil chemotactic protein, small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin)

Species:

Human

Cat. No.:

CRE000C pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$2,700.00 BUY

Description:

Background: CCL11 is a potent eosinophil chemoattractant that was originally purified from bronchoalveolar lavage fluid of guinea pigs sensitized by aerosol challenge with ovalbumin. Human CCL11 cDNA encodes a 97 amino acid residue precursor protein from

Entrez Symbol:

CCL11

Uniprot Name:

Eotaxin

Uniprot ID:

P51671

Data PDF:

CRE000

Source:

E. coli

Molecular Weight:

8.3 kDa

Physical State:

Lyophilized

Formulation:

Lyophilized from a sterile filtered (through a 0.2 ?m filter) concentrated (1 mg/ml) solution in 20 mM PB, pH 7.4 + 150 mM NaCl.

Purity:

>97% by SDS-PAGE and HPLC analyses.

Biological Activity:

ED50: Typically 1-5 ng/ml (As measured by its ability to chemoattract mouse CCR3 transfected BaF3 mouse pro-B cells). Fully biologically active when compared to standard.

Endotoxin Level:

<1EU/�g

Amino Acid Sequence:

GPASVPTTCCFNLANRKIPLQRLESYRRITSGKCPQKAVIFKTKLAKDICADPKKKWVQDSMKYLDQKSPTPKP

Reconstitution:

Centrifuge vial prior to opening. Reconstitute in sterile ddH2O to a concentration of 0.1-1.0 mg/ml. The solution can then be further diluted in other aqueous buffers.

Storage/Stability:

Lyophilized protein is stable at 2-4℃C, but is preferably stored desiccated at -20℃C. After reconstitution, stable for 1 week at 2-4℃C. For long term storage, aliquot and store at -20℃C to -80℃C. Avoid repeated freeze-thaw cy