Recombinant Human LEC/CCL16

Molecule:

CCL16

Synonyms:

CKb12, HCC-4, ILINCK, LCC-1, LEC, LMC, Mtn-1, NCC-4, NCC4, SCYA16, SCYL4, IL-10-inducible chemokine, chemokine CC-4, chemokine LEC, liver CC chemokine-1, liver-expressed chemokine, lymphocyte and monocyte chemoattractant, monotactin-1, new CC chemokine 4

Species:

Human

Cat. No.:

CRL200B pdf (datasheet)

Quantity/Size:

20 μg

Price:

$235.00 BUY

Description:

Recombinant Human CCL16 is a single non-glycosylated polypeptide chain containing 97 amino acids.

Background: CCL16 or HCC-4, also named NCC-4, liver-expressed chemokine (LEC), and lymphocyte and monocyte chemoattractant (LMC), is a novel CC chemokine identified through bioinformatics. HCC-4 cDNA encodes a 120 amino acid (aa) residue precursor protein with a 23 aa residue predicted signal peptide that is cleaved to generate a 97 aa residue mature protein. HCC-4 is distantly related to other CC chemokines, exhibiting less than 30% sequence identity. Among these CC chemokines, HCC-4 has the most similarity to HCC-1. Two potential polyadenylation signals are present on the human HCC-4 gene, and as a result, two transcripts containing approximately 1500 base pairs and 500 base pairs have been detected. HCC-4 is expressed weakly by some lymphocytes, including NK cells, T cells, and some T cell clones. The expression of HCC-4 in monocytes is highly upregulated in the presence of IL-10.

Data PDF:

CRL200

Source:

E. coli

Molecular Weight:

~11.2 kDa

Formulation:

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.

Purity:

>97 % by SDS-PAGE and HPLC analyses.

Biological Activity:

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 10-100 ng/ml.

Endotoxin Level:

Less than 1EU/μg of rHuHCC-4/CCL16 as determined by LAL method.

Amino Acid Sequence:

QPKVPEWVNT PSTCCLKYYE KVLPRRLVVG YRKALNCHLP AIIFVTKRNR EVCTNPNDDW VQEYIKDPNL PLLPTRNLST VKIITAKNGQ PQLLNSQ

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/mL. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

Stable at 2-8°C, but best kept desiccated -20°C. Upon reconstitution, stable for up to 1 week at 2-8°C. For longer term, store in working aliquots below -20°C. Avoid repeated freeze/thaw cycles.