Recombinant Human TARC/CCL17

Molecule:

CCL17

Synonyms:

CCL17, SCYA17, TARC, T cell-directed CC chemokine, small inducible cytokine A17, small inducible cytokine subfamily A (Cys-Cys), member 17, thymus and activation-regulated chemokine

Species:

Human

Cat. No.:

CRT200C pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$2,935.00 BUY

Description:

Recombinant Human CCL17 is a non-glycosylated single chain protein consisting of 71 amino acids.

Background: CCL17/TARC is one of several CC cytokines, proteins characterized by two adjacent cysteines. CCL17 displays chemotactic activity for T lymphocytes, but not monocytes or granulocytes. It binds to chemokine receptors CCR4 and CCR8. CCL17 plays important roles in T cell development in thymus as well as in trafficking and activation of mature T cells. CCL17 is constitutively expressed in thymus, and at a lower level in lung, colon, and small intestine. CCL17 is also transiently expressed in stimulated peripheral blood mononuclear cells.

Entrez Symbol:

CCL17

Data PDF:

CRT200

Source:

E. coli

Molecular Weight:

~8.1 kDa

Formulation:

Lyophilized from a sterile-filtered solution containing 0.1% Trifluoroacetic Acid (TFA).

Purity:

>95% by SDS-PAGE

Biological Activity:

Determined by the ability to chemoattract human T cells at 2-40 ng/ml.

Endotoxin Level:

≤1 EU/µg of protein by kinetic LAL analysis

Amino Acid Sequence:

ARGTNVGREC CLEYFKGAIP LRKLKTWYQT SEDCSRDAIV FVTVQGRAIC SDPNNKRVKN AVKYLQSLER S

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water to a concentration of 0.1 mg/ml. DO NOT VORTEX. Allow several minutes for complete reconstitution. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

Lyophilized product is stable at room temperature for shipping purposes. Upon receipt, store desiccated at -20°C for up to 1 year. Upon reconstitution, the preparation is stable for up to one month at 2-8°C. For long term storage, freeze in working aliquots and store at -20 to -80°C. For maximal stability, dilute to working aliquots in a 0.1% BSA solution. Avoid repeated freeze-thaw cycles.