Recombinant Human MIP-4/CCL18

Molecule:

CCL18

Synonyms:

Chemokine (C-C motif) Ligand 18, MIP-4, CKb7, PARC, AMAC1, DCCK1, SCYA18

Species:

Human

Cat. No.:

CRM410A pdf (datasheet)

Quantity/Size:

2 μg

Price:

$135.00 BUY

Description:

Recombinant Human MIP-4/CCL18 is a single non-glycosylated polypeptide chain containing 69 amino acids.

Background: Human CCL18 is encoded by the CCL18 gene located on the chromosome 17. As also named MIP-4, it shares 61 % sequence identity to human MIP-1α. CCL18 is mainly expressed by lung and some lymphoid tissues such as lymph nodes. It is chemotactic for both activated (CD3+) T cells and nonactivated (CD14-) lymphocytes, but not for monocytes or granulocytes. CCL18 is involved in B-cell migration into B-cell follicles in lymph nodes. CCL18 plays a role in both humoral and cell-mediated immune responses.

Entrez Symbol:

CCL18

Uniprot Name:

C-C motif chemokine 18

Uniprot ID:

P55774

Data PDF:

CRM410

Source:

E. coli

Molecular Weight:

7.8 kDa

Physical State:

Lyophilized

Formulation:

Lyophilized from a sterile filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA).

Purity:

>98% by SDS-PAGE and HPLC.

Biological Activity:

Fully biologically active when compared to standard. The biological activity as determined by its ability to chemoattract primary human PBMC or T lymphocytes is typically in the concentration range of 1.0-8.0 ng/ml.

Specific Activity:

Typically 1 x 106 units/mg

Endotoxin Level:

≤1 EU/µg of protein by kinetic LAL analysis

Amino Acid Sequence:

AQVGTNKELC CLVYTSWQIP QKFIVDYSET SPQCPKPGVI LLTKRGRQIC ADPNKKWVQK YISDLKLNA

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water to a concentration of 0.1 mg/ml. DO NOT VORTEX. Allow several minutes for complete reconstitution.

Storage/Stability:

Upon receipt, store desiccated at -20°C for up to 1 year. Upon reconstitution, the preparation is stable for up to one month at 2-8°C. For long term storage, freeze in working aliquots and store at -20 to -80°C.