Recombinant Human MIP-3 alpha/CCL20

Molecule:

CCL20

Synonyms:

Macrophage Inflammatory Protein 3 alpha, Exodus, CK-beta-4, LARC, MIP-3a, MIP-3-alpha, SCYA20, ST38

Species:

Human

Cat. No.:

CRM408B pdf (datasheet)

Quantity/Size:

20 μg

Price:

$235.00 BUY

Description:

Recombinant Human MIP-3 alpha/CCL20 is a single non-glycosylated polypeptide chain containing 70 amino acids.

Background: MIP-3α/CCL20, also known as LARC (Liver and Activation-regulated Chemokine) and as Exodus, is a CC chemokine that is expressed in the liver, lymph nodes, appendix, PBL and lung and can signal through the CCR6 receptor. MIP-3 alpha is chemotactic towards lymphocytes and dendritic cells. Additionally, it promotes the adhesion of memory CD4+ T cells and inhibits colony formation of bone marrow myeloid immature progenitors.

Entrez Symbol:

CCL20

Uniprot Name:

C-C motif chemokine 20

Uniprot ID:

P78556

Data PDF:

CRM408

Source:

E. coli

Molecular Weight:

~8.0 kDa

Physical State:

Lyophilized

Formulation:

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4 + 100 mM NaCl.

Purity:

>97 % by SDS-PAGE and HPLC analyses.

Biological Activity:

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration range of 10-50 ng/ml.

Endotoxin Level:

Less than 1EU/μg of rHuMIP-3alpha/CCL20 as determined by LAL method.

Amino Acid Sequence:

ASNFDCCLGY TDRILHPKFI VGFTRQLANE GCDINAIIFH TKKKLSVCAN PKQTWVKYIV RLLSKKVKNM

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/mL. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

Stable at 2-8°C, but best kept desiccated -20°C. Upon reconstitution, stable for up to 1 week at 2-8°C. For longer term, store in working aliquots below -20°C. Avoid repeated freeze/thaw cycles.