Recombinant Human MIP-3/CCL23

Molecule:

CCL23

Synonyms:

Macrophage Inflammatory Protein 3, MPIF-1, Ck-beta8

Species:

Human

Cat. No.:

CRM407A pdf (datasheet)

Quantity/Size:

5 μg

Price:

$190.00 BUY

Description:

Recombinant Human MIP-3/CCL23 is a single, non-glycosylated polypeptide chain containing 99 amino acids.

Background: Chemokine (C-C motif) Ligand 23/Macrophage Inflammatory Protein 3 is a CC chemokine that signals through the CCR1 receptor. MIP-3 chemoattracts monocytes, resting T-lymphocytes and neutrophils, but does not chemoattract activated lymphocytes. Additionally, MIP-3 has been shown to inhibit colony formation of bone marrow myeloid immature progenitors. Alternative splicing of the MPIF1 gene results in two mRNAs that encode a short (CKbeta8) and a long (CKbeta81) isoform of the chemokine. CKbeta8 cDNA encodes a 120 amino acid (aa) residue precursor protein with a putative 21 aa residue signal peptide that is cleaved to generate a 99 aa residue mature CKbeta8 (aa 22 120). Additional N terminal processing of the 99 aa residue variant can generate a 75 aa residue CKbeta8 (aa 46 120) that is significantly more active than the 99 aa residue variant.

Entrez Symbol:

CCL23

Uniprot Name:

C-C motif chemokine 23

Uniprot ID:

P55773

Data PDF:

CRM407

Source:

E. coli

Molecular Weight:

~11.4 kDa

Physical State:

Lyophilized

Formulation:

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.

Purity:

>97 % by SDS-PAGE and HPLC analyses.

Biological Activity:

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration of 10-50 ng/ml.

Endotoxin Level:

Less than 1EU/μg of rHuMIP-3/CCL23 as determined by LAL method.

Amino Acid Sequence:

RVTKDAETEF MMSKLPLENP VLLDRFHATS ADCCISYTPR SIPCSLLESY FETNSECSKP GVIFLTKKGR RFCANPSDKQ VQVCMRMLKL DTRIKTRKN

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/mL. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

Stable at 2-8°C, but best kept desiccated -20°C. Upon reconstitution, stable for up to 1 week at 2-8°C. For longer term, store in working aliquots below -20°C. Avoid repeated freeze/thaw cycles.