Recombinant Human MIP-1 alpha/CCL3

Molecule:

CCL3

Synonyms:

Macrophage Inflammatory Protein 1-alpha, CCL3, LD78-alpha, MIP1A, MIP-1-alpha, SCI, SCYA3, SIS-beta, TY5

Species:

Human

Cat. No.:

CRM400C pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$2,700.00 / Special Price: $1,640.00 BUY

Description:

Recombinant Human MIP-1 alpha/CCL3 contains 70 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.

Background: CCL3, also known as macrophage inflammatory protein-1α (MIP-1α), is a cytokine belonging to the CC chemokine family that is involved in the acute inflammatory state in the recruitment and activation of polymorphonuclear leukocytes. Studies showed CCL3 can bind to CCR1, CCR4 and CCR5. MIP-1α can induce a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). Recombinant human MIP-1α shares approximately 73 %, 74 % and 92 % amino acid sequence homology with the murine, rat and rhesus macaque protein.

Entrez Symbol:

CCL3

Uniprot Name:

C-C motif chemokine 3

Uniprot ID:

P10147

Data PDF:

CRM400

Source:

E. coli

Molecular Weight:

~7.8 kDa

Physical State:

Lyophilized

Formulation:

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl.

Purity:

>96 % by SDS-PAGE and HPLC analyses.

Biological Activity:

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 1.0-10 ng/ml.

Endotoxin Level:

<1 EU/μg as determined by LAL method.

Amino Acid Sequence:

ASLAADTPTA CCFSYTSRQI PQNFIADYFE TSSQCSKPGV IFLTKRSRQV CADPSEEWVQ KYVSDLELSA

Reconstitution:

Centrifuge vial prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C.

Storage/Stability:

This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C.