Tumor Necrosis Factor Suerfamily Member 5, CD154, CD40L, TRAP, HIGM1, IGM, IMD3, LY-62, T-BAM, TNFSF5
Recombinant human soluble CD40 Ligand is a non-glycosylated polypeptide chain containing 149 amino acids.
Background: CD40 ligand, CD40L (also known as CD154, TRAP or gp39), is a 261 amino acid type II transmembrane glycoprotein belonging to the TNF family, CD40L is expressed predominantly on activated CD4+ T lymphocytes, and also found in other types of cells, like NK cells, mast cells, basophils and eosinophils. Human CD40L shares 78% amino acid identity with its mouse counterpart. The receptor of CD40L is CD40, a type I transmembrane glycoprotein belonging to the TNF receptor family. CD40 is expressed on B lymphocytes, monocytes, dendritic cells and thymic epithelium. Although all monomeric, dimeric and trimeric forms of soluble CD40L can bind to CD40, the trimeric form of soluble CD40L has the most potent biological activity through oligomerization of cell surface CD40, a common feature of TNF receptor family members. CD40L mediates a range of activities on B cells including induction of activation-associated surface antigen, entry into cell cycle, isotype switching and Ig secretion and memory generation. CD40-CD40L interaction also plays important roles in monocyte activation and dendritic cell maturation.
Lyophilized from a sterile-filtered solution in 10 mM sodium phosphate, pH 7.5
>95.0% by HPLC and SDS-PAGE
Fully biologically active when compared to standard. The ED50 as determined by dose-dependent IL-8 production by human PBMCs, typically 5-10 ng/ml. As determined by proliferation of human PBMCs, typically 1 µg/ml.
Typically 2 x 10e5 units/mg (by IL-8 production) or 1 x 10e3 units/mg (by PBMC proliferation).
≤1 U/µg of protein by kinetic LAL analysis (50% confidence range).
Amino Acid Sequence:
MQKGDQNPQI AAHVISEASS KTTSVLQWAE KGYYTMSNNL VTLENGKQLT VKRQGLYYIY AQVTFCSNRE ASSQAPFIAS LWLKSPGRFE RILLRAANTH SSAKPCGQQS IHLGGVFELQ PGASVFVNVT DPSQVSHGTG FTSFGLLKL
Centrifuge vial prior to opening. Add sterile distilled water to a concentration of 0.1 mg/ml. DO NOT VORTEX. Allow several minutes for complete reconstitution. Further dilutions should be made in appropriate buffered solutions.
Lyophilized protein is stable at room temperature for shipping. On receipt, store desiccated at -20°C. After reconstitution, stable for one month at 2-8°C. For longer term, store aliquots at -20°C to -80°C. Avoid repeated freeze-thaw cycles.