Recombinant Human CDK9/CCNT1 complex (CycT1 complex) His

Molecule:

CDK9/CCNT1 complex

Synonyms:

TAK, C-2k, CTK1, CDC2L4, PITALRE, CDC2-related kinase, cell division protein kinase 9, serine, threonine protein kinase PITALRE, serine, threonine-protein kinase PITALRE, cell division cycle 2-like protein kinase 4 , CCNT, CYCT1, HIVE1, cyclin-T, cyclin T1b, cyclin C-rel

Species:

Human

Cat. No.:

CSI10994 pdf (datasheet)

Quantity/Size:

10 μg

Price:

$585.00 BUY

Description:

Product: Recombinant Human full length CDK9/Cyclin T1 complex, Histidine-tagged, expressed in insect cells. No special measures were taken to activate this kinase. Mass Spectrometry: The enzyme preparation was subjected to tryptic digestion followed by LC

Concentration:

0.26 mg/ml (total protein as measured using the Bradford protein assay with BSA as a standard).

Entrez Symbol:

CDK9 | CCNT1

Uniprot Name:

Cell division protein kinase 9 | Cyclin-T1

Uniprot ID:

P50750 | O60563

EC number:

CDK9 - 2.7.11.22 | 2.7.11.23

Data PDF:

CSI10994

Genbank Accession No:

NP_001252 | NP_001231

Source:

Insect cells

Molecular Weight:

46.9 kDa | 84.8 kDa

Tag:

His

Physical State:

Frozen liquid

Formulation:

Sterile filtered liquid (Frozen) in 50 mM Tris Buffer, pH 7.5 + 150 mM NaCl + 0.5 mM EDTA + 0.02% Triton� X-100 + 2 mM DTT + 50% Glycerol.

Purity:

>70% by SDS-PAGE analysis.

Specific Activity:

59 nmol/min/mg (phosphate transferred to CDK7/9tide substrate (YSPTSPSYSPTSPSYSPTSPS-KKKK) per minute per mg of total protein at 30�C. Activity determined at a final protein concentration of 3.33 ?g/ml).

Amino Acid Sequence:

MSYYHHHHHHDYDIPTTENLYFQGITSLYKKAGTMAKQYDSVECPFCDEVSKYEKLAKIGQGTFGEVFKARHRKTGQKVALKKVLMENEKEGFPITALREIKILQLLKHENVVNLIEICRTKASPYNRCKGSIYLVFDFCEHDLAGLLSNVLVKFTLSEIKRVMQMLLNGLYYIHRNKILHRDMKAANVLITRDGVLKLADFGLARAFSLAKNSQPNRYTNRVVTLWYRPPELLLGERDYGPPIDLWGAGCIMAE

Storage/Stability:

Frozen liquid protein is stable at -80℃C for 6 months from date of purchase. For long term storage, aliquot and store at -80℃C. Avoid repeated freeze-thaw cycles.

Application notes:

The dilution buffer described is recommended for the radiometric assay format. For high throughput applications such as PolarScreen, Z'-LYTE, and LanthaScreen, modifications to this buffer are needed.