Recombinant Human CX3CL1/Fractalkine

Molecule:

CX3CL1

Synonyms:

Fractalkine, Neurotactin, CXC3, CXC3C, NTN, NTT, SCYD1

Species:

Human

Cat. No.:

CRF200A pdf (datasheet)

Quantity/Size:

5 μg

Price:

$190.00 BUY

Description:

Recombinant human CX3CL1 is a single, non-glycosylated poly-peptide chain containing 76 amino acids.

Background: Fractalkine, also named neurotactin, is a novel chemokine recently identified through bioinformatics. Fractalkine has a unique CX3-C cysteine motif near the amino-terminus and is the first member of a fourth branch of the chemokine superfamily. Unlike other known chemokines, fractalkine is a type 1 membrane protein containing a chemokine domain tethered on a long mucin-like stalk. Human fractalkine cDNA encodes a 397 amino acid (a.a.) residue membrane protein with a 24 aa residue predicted signal peptide, a 76 aa residue chemokine domain, a 241 aa residue stalk region containing 17 degenerate mucin-like repeats, a 19 aa residue transmembrane segment and a 37 aa residue cytoplasmic domain. The extracellular domain of human fractalkine can be released, possibly by proteolysis at the dibasic cleavage site proximal to the membrane, to generate soluble fractalkine. The soluble chemokine domain of human fractalkine was reported to be chemotactic for T cells and monocytes while the soluble chemokine domain of mouse fractalkine was reported to chemoattract neutrophils

Data PDF:

CRF200

Source:

E. coli

Molecular Weight:

~8.6 kDa

Formulation:

Lyophilized from a 0.2 μm sterile filtered solution in 20 mM PBS, pH 7.4 + 50 mM NaCl

Purity:

>97% by SDS-PAGE and HPLC analyses

Biological Activity:

Fully biologically active when compared to standard. The biological activity determined by achemotaxis bioassay using human T-lymphocytes is in a concentration of 5.0-10 ng/ml.

Endotoxin Level:

Less than 1EU/μg as determined by LAL method.

Amino Acid Sequence:

QHHGVTKCNI TCSKMTSKIP VALLIHYQQN QASCGKRAII LETRQHRLFC ADPKEQWVKD AMQHLDRQAA ALTRNG

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

This lyophilized preparation is stable at 2-4°C, but should be kept desiccated at -20°C for long term storage. Upon reconstitution, the preparation is stable for up to one week at 2-4°C.