Recombinant Human CXCL1/GRO-alpha

Molecule:

CXCL1

Synonyms:

FSP, GRO1, GROa, MGSA, MGSA-a, NAP-3, SCYB1, GRO1 oncogene (melanoma growth stimulating activity, alpha), GRO1 oncogene (melanoma growth-stimulating activity), MGSA alpha, chemokine (C-X-C motif) ligand 1, fibroblast secretory protein, melanoma growth sti

Species:

Human

Cat. No.:

CRG500C pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$2,700.00 / Special Price: $1,640.00 BUY

Description:

The three GRO cDNAs encode 107 amino acid precursor proteins from which the N-terminal 34 amino acid residues are cleaved to generate the mature GROs. There are no potential N-linked glycosylation sites in the amino acid sequences. GRO expression is inducible by serum or PDGF and/or by a variety of inflammatory mediators, such as IL-1 and TNF, in monocytes, fibroblasts, melanocytes and epithelial cells. In certain tumor cell lines, GRO is expressed constitutively. Similar to other alpha chemokines, the three GRO proteins are potent neutrophil attractants and activators. In addition, these chemokines are also active toward basophils. All three GROs can bind with high affinity to the IL-8 receptor type B.

Recombinant Human GRO-alpha/CXCL1 is a single, non-glycosylated polypeptide chain containing 73 amino acids.

Entrez Symbol:

CXCL1

Uniprot Name:

Growth-regulated alpha protein

Uniprot ID:

P09341

Data PDF:

CRG500

Source:

E. coli

Molecular Weight:

~7.8 kDa

Formulation:

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4 + 150 mM NaCl.

Purity:

>97% by SDS-PAGE and HPLC analyses.

Biological Activity:

Fully biologically active when compared to standard. The ED50 determined by a chemotaxis bioassay using human peripheral blood neutrophils is <50 ng/ml.

Endotoxin Level:

<1 EU/μg as determined by LAL method.

Amino Acid Sequence:

ASVATELRCQ CLQTLQGIHP KNIQSVNVKS PGPHCAQTEV IATLKNGRKACLNPASPIVK KIIEKMLNSD KSN

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

This lyophilized preparation is stable at 2-8°C, but should be kept desiccated at -20°C for long term storage. Upon reconstitution, the preparation is stable for up to one week at 2-8°C.